促性腺激素释放激素类似物对小鼠种植期子宫内膜白血病抑制因子、同源框基因a10及胞饮突的影响

Impact of gonadotropin-releasing hormone analogous on leukaemia inhibitory factor, homeobox gene a10 and pinopodes in mouse endometrium during implantation window

目的探讨促性腺激素释放激素(GnRH)类似物对小鼠种植期子宫内膜白血病抑制因子(LIF)、同源框基因(HOX)a10及胞饮突的影响。方法将8周龄雌性小鼠采用随机数字表法随机分为3组,各组20只,于妊娠第3.5日分别腹腔注射短效GnRH激动剂(GnRH-a)(GnRH-a组)、GnRH拮抗剂(GnRH-A)(GnRH-A组)、生理盐水(对照组),于妊娠第4.5日取小鼠血测雌孕激素、人绒毛膜促性腺激素(hCG)水平;小鼠子宫内膜扫描电子显微镜观察种植窗期胞饮突,RT-PCR检测LIF mRNA、HOXa10 mRNA的表达。结果妊娠第4.5日3组孕酮、雌二醇、hCG水平差异均无统计学意义(P>0.05)。种植期GnRH-a组、对照组子宫内膜胞饮突发育优于GnRH-A组。GnRH-a组小鼠着床期子宫内膜LIF mRNA(1.26±0.10)和HOXa10 mRNA(1.30±0.12)表达水平明显高于对照组(1.20±0.06,1.23±0.09)(P=0.043、P=0.028),GnRH-A组小鼠着床期子宫内膜LIF mRNA(1.14±0.07)和HOXa10 mRNA(1.15±0.08)表达水平明显低于对照组(P=0.002、P=0.007)。结论黄体期GnRH-a有益于种植期子宫内膜LIF 、HOXa10表达及胞饮突发育,可提高子宫内膜容受性,而GnRH-A则反之。

Objective To evaluate the effects of gonadotropin-releasing hormone (GnRH) analogous on the expression of leukaemia inhibitory factor (LIF), homeobox gene a10 (HOXa10) and pinopodes in mouse endometrium during the implantation window. Methods Sixty female mice were divided into three groups equally and randomly, which were abdominal injected by GnRH agonist (GnRH-a)(GnRH-a group), GnRH antagonist (GnRH-A)(GnRH-A group), normal saline (control group) individually 3.5 d after pregnancy (day 3.5), and serum hormone test was performed on day 4.5;mouse uteri were collected on day 4.5 to defect the expression ofLIF mRNA andHOXa10 mRNA by RT-PCR, and pinopodes were observed by electronic microscopy. Results There were no significant differernces in estrodial, progesterone and human chorionic gonadotropin (hCG) among three groups. Pinopodes grew better in GnRH-a group and control group than in GnRH-A group. Theexpressions ofLIF mRNA(1.26±0.10)andHOXa10 mRNA(1.30±0.12)in GnRH-a group were significantly higher than those in control group(1.20±0.06, 1.23±0.09)(P=0.043,P=0.028), and the expressions ofLIF mRNA(1.14±0.07)andHOXa10 mRNA(1.15±0.08)in GnRH-A group were significantly lower than those in control group (P=0.002,P=0.007). Conclusion GnRH-a during luteal phase can increase the expression ofLIF ,HOXa10 in endometrium and formation of pinopode, improve endometrium receptivity, while GnRH-A has the opposite function.