白囊耙齿菌子实体提取物对小鼠慢性肾小球肾炎的预防与治疗

Prevention and treatment of chronic glomerulonephritis in mice by administrating Irpex lacteus fruiting body extract

本文对白囊耙齿菌子实体不同提取物防治慢性肾小球肾炎进行研究,采用腺嘌呤灌胃法建立小鼠慢性肾小球肾炎模型,探究白囊耙齿菌子实体水提取物和醇提取物的高、中、低剂量组对慢性肾小球肾炎的预防作用及不同提取物的高剂量组对慢性肾小球肾炎的治疗作用,并对小鼠体重进行测量,检测尿蛋白(UP)、尿素氮(BUN)、肌酐(Cr)、总胆固醇(TC)、超氧歧化酶(SOD)、一氧化氮(NO)、丙二醛(MDA)、白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)指标,分析肾、肝、脾、胸腺的病理变化。结果表明,水提取物中剂量(240mg/kg)能显著降低小鼠尿蛋白含量和肾组织中炎症因子含量,使血清生化指标维持正常,并对免疫器官有保护作用;醇提取物高剂量(200mg/kg)对慢性肾小球肾炎有治疗作用,但对免疫器官保护作用较弱。研究表明,白囊耙齿菌子实体水提取物中剂量具有预防慢性肾小球肾炎的作用且效果优于阳性药肾炎康胶囊,具有开发利用潜力,机制有待于进一步研究。

Prevention and treatment of chronic glomerulonephritis of mice through administrating fruiting body extract of Irpex lacteus are investigated. Model of chronic nephritis of mice was established by the method of oral administration of adenine. Preventive effect of high, medium, low doses and therapeutic effect of high dose of water extract and alcohol extract of the fungal fruiting body on chronic glomerulonephritis were observed. The body weight and the indexes of UP, BUN, Cr, TC, SOD, NO, MDA, IL-6, IL-1β and TNF-α of the mice were monitored. Pathological changes in kidney, liver, spleen and thymus were analysed. The results show that the medium dose of 240 mg/kg of the water extract can significantly reduce urine protein content and inflammatory factor content in kidney tissue, keeping the blood biochemical indicators normal, and having protective effect on immune organs. The high dose of 200 mg/kg of alcohol extract can prevent chronic nephritis, but weakly protect immune organs. The results suggest that the medium dose of water extract is superior in preventing glomerulonephritis, and has potential for development and utilization. The pharmacological mechanism of Irpex lacteus fruiting body extract needs further study.