高效液相色谱法检测不同类型消毒剂中度米芬含量的方法研究

Study on the content determination of domiphen in different form of disinfection products by HPLC

目的建立测定不同类型消毒产品中度米芬含量的高效液相色谱方法。方法在Sepax HP-SCX (4. 6×250 mm,5μ,磺化交联的苯乙烯-二乙烯基苯共聚物为填充剂)离子交换树脂色谱柱上,以甲醇+0. 05 mol/L醋酸钠溶液(80:20)为流动相,流量为1. 0 ml/min,柱温为40℃,检测波长为274 nm条件下,测定度米芬含量。结果度米芬的浓度范围为0. 101 8～2. 035 1 mg/ml时,峰面积与浓度呈良好的线性关系(r=0. 999 8,n=6),平均回收率为106. 70%,RSD为2. 25%。结论该方法准确简便、通用且专属性强,有助于检测和判断消毒产品中度米芬违规添加问题,能够为填补《消毒技术规范》中消毒剂产品度米芬含量测定方法的空白提供技术支持。

Objective A high performance liquid chromatography( HPLC) method for the determination of domiphen in different form of disinfection products was developed.Methods The sample was separated on Sepax HP-SCX ion exchange resin column using methol + 0.05 mol/L sodium acetate( 80 ∶ 20,v/v) as the mobile phase at a flow rate of 1.0 ml/min.The column temperature was set at 40 ℃,and domiphen was determined with UV detection at a wavelength of 274 nm.Results A good linearity between the concentrations of domiphen and their peak areas was shown from 0.101 8 to2.035 1 mg/ml( r = 0.999 8,n = 6),and the recovery of this method was 106.70% with RSD of 2.25 %.Conclusion The method is convenient,accurate,specific and rapid for routine analysis.This method will be helpful for detecting and judging the illegal addition of domiphen products,and it can provide technical support for filling the blank of the determination method of domiphen in disinfection products in the Technical Standard for Disinfection of China.