昆明小鼠放射性肺炎病理观察

Pathological Changes of Radiation Pneumonia in Kunming Mice

目的阐明昆明小鼠放射性肺炎发生发展过程的病理变化。方法采用6MV的X射线20Gy单次全胸照射造模,分别于照射后第7、14、28天取材进行HE染色、病理观察,并应用Image-Pro Plus图像分析系统对肺泡壁厚度、肺泡腔面密度、肺间质面密度进行定量测定分析。结果辐照后随时间延长,肺泡萎缩、肺间隔增宽、炎细胞浸润程度加重,28 d后可见肺泡塌陷实变,轻度纤维化改变。肺泡壁厚度、肺泡腔面密度、肺间质面密度测量结果差异极显著。结论采用上述方法复制的昆明小鼠放射性肺炎模型,病理变化过程符合放射性肺炎病程发展。

Objective To clarify development of the pathological process of radiation pneumonia. Method Using 6 MV X-ray, of which the cumulative dose is 20 Gy, irradiates the full chest once. The hematoxylin-eosin staining was conducted respectively on the 7 th day, 14 th day and 28 th day after irradiation for pathological study. In addition, the Image-Pro plus analysis system was applied to quantitatively analyze thickness of alveolar walls, and surface mass density of alveolar spaces and interstitial tissue. Result Alveolar atrophy, alveolar septum widening and inflammatory cell infiltration were observed along with the development process. The collapsed or consolidated alveolus and mild pulmonary fibrosis were observed after 28 days. Furthermore, the differences of thickness of alveolar walls and surface mass density of alveolar spaces and interstitial tissue were very significant. Conclusion Radiation pneumonia model in Kunming Mouse could be replicated successfully by the mentioned method, of which the pathological changes are in accordance with the clinical course.