摘要
目的研究雷公藤红素对人肠癌细胞CCL-244增殖、干性的作用及可能的机制。方法体外培养人肠癌细胞CCL-244,经不同浓度雷公藤红素处理,采用MTT法检测雷公藤红素对细胞增殖活性的影响;克隆形成法测定雷公藤红素对细胞克隆形成能力的影响;RT-PCR法测定雷公藤红素对CD133、CD44和Oct4基因表达的影响;免疫印迹法测定雷公藤红素对CD133、CD44和AKT信号通路蛋白表达的影响。结果雷公藤红素可明显抑制人肠癌CCL-244细胞的增殖;与对照组比较,雷公藤红素处理后可明显抑制肠癌细胞CCL-244的克隆形成;降低干性相关基因CD133、CD44和Oct4的表达;降低干性相关蛋白CD133和CD44的表达;抑制pAKT的磷酸化表达。结论雷公藤红素可抑制人肠癌CCL-244细胞增殖和克隆形成,这可能与抑制干性相关基因和蛋白CD133、CD44等的表达、抑制AKT信号通路有关。
Objective To investigate the effect and possible mechanism of celastrol on proliferation and stemness of human colorectal cancer CCL-244 cells. Methods After CCL-244 cells were incubated with various- concentrations of celastrol,the cell proliferation was examined by MTT assay,while the colony formation was measured by colony formation assay. The expression of genes(CD133,CD44 and Oct4)was measured by semi- quantitative RT-PCR. Moreover,the expression of CD133,CD44,pAKT and AKT proteins was detected by west- ern blot assay. Results Compared with the control group,the celastrol of different concentrations could significantly inhibit the CCL-244 cells proliferation,suppress the colony formation,lower the expression rates of CD133、CD44 and Oct4 mRNAs and decrease the expression of CD133,CD44 and pAKT proteins. Conclusion Celastrol may inhibit CCL-244 cell proliferation and colony formation by inhibiting the expression of CD133,CD44 and pAKT mRNAs and proteins.
作者
苏兰娣
李芹
江秀玲
罗雪
彭建明
SU Landi;LI Qin;JIANG Xiuling;LUO Xue;PENG Jianming(Medical College of Yangzhou Polytechnic College,Yangzhou 225009,China)
出处
《实用医学杂志》
CAS
北大核心
2019年第6期860-863,共4页
The Journal of Practical Medicine
基金
江苏省卫生厅卫生职业技术教育科研资助项目基金(编号:NJ201210)
关键词
肠癌
雷公藤红素
干性
colorectal cancer
celastrol
stemness