CLDND1异常表达与结直肠癌发生的相关性

Relationship between abnormal expression of CLDND1 and colorectal cancer

目的确定含有claudin结构域蛋白1(claudin domain containing 1,CLDND1)RNA的表达变化与结直肠癌发生发展的相关性。方法收集35例患者的结直肠癌及其癌旁组织,抽提总RNA,应用qRT-PCR技术检测并比较CLDND1转录情况。构建CLDND1 RNA真核表达载体,转染SW480细胞过表达CLDND1 RNA后,用MTT法检测SW480细胞的增殖能力变化。同时合成反义RNA干扰CLDND1 RNA在SW480细胞中的表达,用流式细胞技术检测干扰后SW480细胞凋亡情况变化。结果 qRT-PCR结果显示结直肠癌组织中CLDND1的转录水平明显高于癌旁组织,差异有统计学意义;过表达CLDND1 RNA的SW480细胞的增殖能力明显增强;下调SW480细胞中CLDND1基因的转录,其凋亡率明显增高,差异有统计学意义。结论 CLDND1基因的异常转录与结直肠癌的发生发展具有相关性,其可以抑制结肠癌细胞的凋亡。因此,CLDND1 RNA可能成为结直肠癌临床诊断的分子标志物和治疗靶点。

Objective To study the effect of expression change of CLDND1(claudin domain containing 1) on the occurrence and development of colorectal cancer and to explore its role and mechanism in the occurrence of colorectal cancer. Methods Thirty five pairs of colorectal cancer tissue and its adjacent tissue were collected and the expression of CLDND1 mRNA was detected by quantitative real time polymerase chain reaction(qRT?PCR). The effect of CLDND1 on SW480 was examined by comparing the apoptosis rate after CLDND1 was over expressed with eukaryotic expression plasmid or down-regulated with antisense RNA. Results qRT-PCR results showed that the expression of CLDND1 mRNA in coloratal cancer tissue was significantly higher than that in adjacent tissues. The apoptosis rate of SW480 cells was significantly higher than that of controls when CLDND1 expressionwas down-regulated. Conclusion CLDND1 is correlated with the occurrence of colorectal cancer. It may be involved in the occurrence and development of colorectal cancer by inhibiting apoptosis,and has the potential to be used as a molecular marker for clinical diagnosis of colorectal cancer.