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苹果组培外植体消毒及培养基优化试验研究 被引量:3

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摘要 本文主要研究苹果外植体的消毒方法,以及对诱导培养基的优化。结果表明:酒精消毒时间为15 s,升汞消毒时间为8 min时,外植体的污染率、褐化率最低,成活率最高,最适宜的茎尖培养基为2/3MS+1.0 mg/L 6-BA+0.1 mg/L NAA+20 g/L蔗糖+琼脂6 g/L, p H5.8。
出处 《现代农村科技》 2019年第4期55-56,共2页
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