摘要
目的研究SureID~?Compass试剂盒性能指标,建立适合其特点的测试方案。方法选择合适的DNA标准物质,从标准品灵敏度、混合样本、种属特异性、不同检材适应性、降解样本和含抑制剂样本的耐受性、不同仪器间一致性、峰值均衡性、反复冻融稳定性等8个方面对试剂盒进行测试。结果经检测, SureID~?Compass试剂盒灵敏度较高,对混合样本、酶切降解以及含抑制剂的样品具有一定的检验能力,种属特异性、适应性和峰值均衡性也均较好,所选择的DNA标准物质适用于该试剂盒测试。结论本测试方案适用于同步扩增常染色体和Y染色体STR试剂盒的性能指标测试。
Objective To test the technical parameters of SureID^■ Compass Amplification Kit by establishing an adaptive performance testing procedure. Methods The kit was validated with the selected adaptive DNA reference materials on the items of sensitivity, mixed samples, species specificity, adaptability, survivability, consistency, peak height balance and stability. Results SureID^■ Compass Kit is adaptive to the mixed, degraded and inhibited samples, shown of high sensitivity. It is also appropriate with the items of species specificity, adaptability and peak height balance. The selected DNA reference materials are suitable to this test and the kit. Conclusions The performance-testing procedure established here is applicable to verify an eligible kit for its synchronizing amplification of autosomal and Y chromosomal STRs.
作者
田芳
王旭
王顺霞
张庆霞
焦章平
路志勇
TIAN Fang;WANG Xu;WANG Shunxia;ZHANG Qingxia;JIAO Zhangping;LU Zhiyong(Forensic Science Service of Shijingshan Branch of Beijing Public Security Bureau, Beijing 100043, China;Forensic Science Service of Beijing Public Security Bureau, Beijing 100192, China)
出处
《刑事技术》
2019年第2期175-179,共5页
Forensic Science and Technology