浙江大盘山华顶杜鹃菌根真菌的分离和鉴定

Isolation and Identification of Mycorrhizal fungi in Rhododendron huadingense Root in Zhejiang Dapanshan National Nature Reserve

于2016年5月在浙江省大盘山国家级自然保护区的3处采样地,每处采集5株华顶杜鹃Rhododendron huadingense的1～2支侧根,培养分离华顶杜鹃的菌根真菌,然后结合菌落形态特征和r DNAITS序列分析进行鉴定。结果表明:华顶杜鹃根系内共分离到165个菌株,分为22个菌落形态类型;结合r DNA ITS序列分析,最终分为20个不同的菌种类型,其中19个菌种类型属于子囊菌门Ascomycota,包括8个属于粪壳菌纲Sordariomycetes和6个属于锤舌菌纲Leotiomycetes;3个优势菌种分别为Phialocephala fortinii,Ilyonectria radicicola和某粪壳菌纲真菌的近缘种,分别占菌株总数的41.82%,22.42%和10.91%;其余17个菌种类型合计只占总数的24.85%。

Three sample plots were established in Dapanshan National Nature Reserve, Zhejiang province in May of 2016. 5 Rhododendron huadingense were selected for 1-2 lateral roots each. Ericoid mycorrhiza (ERM) was cultured from collected roots and studied by morphological characteristics and molecular identification of rDNA ITS sequence. The result showed that 165 mycorrhizal fungal strains were isolated which were classified into 22 types based on colony characteristics. rDNA ITS demonstrated that these mycorrhizal fungi could be identified into 20 species, among them, 19 species belonging to Ascomycota, including 8 Sordariomycetes, 6 Leotiomycetes. The dominant 3 species were Phialocephala fortinii, Ilyonectria radicicola and Sordariomycetes sp., respectively accounting for 41.82 %, 22.42% and 10.91% of the total strains. The lest 17 species occupied only 24.9%.