MiR-514与胃癌细胞凋亡的关系及机制

Relationship between miR-514 and apoptosis of gastric cancer and related mechanism

目的探讨微小RNA-514(microRNA-514,miR-514)与胃癌细胞凋亡的关系及机制。方法实时定量PCR检测80例胃癌组织及癌旁正常胃黏膜组织中miR-514的表达情况,分析miR-514与患者各病理参数之间的关系。应用miR-514模拟物转染人胃癌细胞株MGC803,检测转染前后细胞凋亡率及凋亡相关基因的情况。结果胃癌组织中miR-514水平(0. 3619±0. 2103)明显低于癌旁正常胃黏膜(0. 6943±0. 3032)(t=-8. 0573; P<0. 001); miR-514表达与肿瘤的淋巴结转移有关(t=-2. 5620,P=0. 012 3); miR-514模拟物转染人胃癌细胞株MGC803后细胞的活性明显降低而凋亡率明显增高(P<0. 05);转染后凋亡相关基因Bcl-2、x IAP的mRNA和蛋白表达明显降低,而Bax的mRNA和蛋白表达明显增高(P <0. 05)。结论胃癌组织中miR-514的表达减弱,miR-514可能通过调节一些凋亡基因表达而参与肿瘤的凋亡过程。

Objective To investigate the relationship between microRNA-514( miR-514)and apoptosis of gastric cancer and related mechanism. Methods Expression of miR-514 in gastric cancer tissues and cancer-adjacent normal tissues from 80 patients were detected with real-time PCR,and relationship between miR-514 and clinicopathological parameters was investigated. miR-514 mimics was utilized to transfected into gastric cancer cell line MGC803,then variation of apoptosis rate and related genes was detected. Results Level of miR-514 was lower in gastric cancer tissues( 0.3619 ± 0.2103) than that in cancer-adjacent normal tissues( 0.6943 ± 0.3032)( t =-8.0573,P < 0.001). Expression of miR-514 was related with lymph node metastasis( t =-2.5620,P = 0.0123). After transfection of miR-514 mimics,cell activity was decreased,while apoptotic rate of MGC803 increased significantly( P < 0. 05);mRNA and protein of Bcl-2,xIAP were decreased significantly,while Bax increased significantly( P<0. 05). Conclusion Reduction of miR-514 existed in gastric cancer tissues,which may be invovled in apoptosis of gastric cancer cells by regulating apoptosis related genes.