肺闭宁颗粒止咳、抗炎及促进PM2.5颗粒物排出作用的研究

Antitussive,anti-inflammatory effects and promotion on excretion of ultrafine particles in PM2.5 of Feibining Granule

[目的]考察肺闭宁颗粒在止咳、抗炎以及对促进PM2.5排出方面的药效学作用研究,为其临床用药提供数据支持。[方法]采用二氧化硫引咳小鼠模型、内毒素诱导的肺损伤小鼠模型以及斑马鱼PM2.5排出模型,给予不同浓度的肺闭宁颗粒干预后,观察其止咳、抗炎以及对纳米颗粒从斑马鱼体内排出进入肠道的影响。[结果]肺闭宁颗粒中(3.69 g/kg)、高(7.38 g/kg)剂量组可明显改善二氧化硫引咳模型小鼠咳嗽潜伏期时间及咳嗽次数;可降低内毒素诱导的肺损伤小鼠肺灌流液中白细胞介素-6(IL-6)浓度(P<0.05);在斑马鱼PM2.5超细颗粒物排出模型中,肺闭宁颗粒125 mg/mL时,纳米活性炭排出发生率为63.3%,与模型对照组(16.7%)比较有统计学差异(P<0.05)。[结论]肺闭宁颗粒具有止咳、抗炎的作用,此外在促进PM2.5超细颗粒物排出、减少PM2.5对机体的损伤方面也有一定的效果,提示其在对抗雾霾天气引起的呼吸道系统疾病治疗中具有一定作用。

[Objective] To investigate the antitussive,anti-inflammatory effects and promotion on excretion of ultrafine particles in PM2.5 of Feibining Granules.[Methods] A mouse model of cough caused by sulphur dioxide,a mouse model of endotoxin-induced lung injury, and a zebrafish model of the ultrafine particles in PM2.5 excretion were given. After intervention with different concentrations of Feibining Granules,the antitussive and anti-inflammatory effects and the promotion of ultrafine particles in PM2.5 excretion were observed.[Results] The medium and high dose groups of Feibining Granules could significantly improve the cough latency and cough frequency of sulphur dioxide cough model mice. Both of the groups could reduce lung perfusion of mice with endotoxin-induced lung injury and the concentration of IL-6 in the liquid (P<0.05).In the zebrafish model,the incidence of nano-activated carbon secretion was 63.3% when the concentration was 125 mg/mL,compared with the model control group (16.7%). There was a statistically significant difference (P<0.05).[Conclusion] Feibining Granules have antitussive and anti-inflammatory effects,and also have certain effects in promoting the excretion of ultrafine particles in PM2.5 and reducing the damage of PM2.5. It suggests that it acts a certain role in the treatment of respiratory diseases caused by smog weather.