米诺环素对大鼠闭合性脊髓损伤的神经保护作用

Effect of Minocycline on Acute Closed Spinal Cord Injury in Rats

目的探讨米诺环素对大鼠急性闭合性脊髓损伤后继发性损伤的保护作用。方法 24只Sprague-Dawley雄性大鼠随机分为盐水组(n=8)、氯化镁组(n=8)和米诺环素组(n=8)。球囊压迫大鼠脊髓背侧,制备闭合性脊髓损伤模型,动物磁共振选取制模成功大鼠。各组于术后1～7 d静脉注射相应药物。术后2～31 d行BBB评分。术后31 d行运动诱发电位和感觉诱发电位检测,取脊髓损伤段行Luxol Fast Blue染色观察继发性损伤面积。结果动物磁共振显示T_(10)脊髓T_2低信号。术后17～31 d,米诺环素组BBB评分高于盐水组(P <0.05)。米诺环素组运动诱发电位振幅高于盐水组(P <0.05),继发性损伤面积小于盐水组(P <0.05)。结论米诺环素在脊髓急性损伤后,对继发性损伤有保护作用。

Objective To investigate the neuroprotective effect of minocycline on the secondary injury after acute closed spinal cord injury in rats.Methods A total of 24 Sprague-Dawley male rats were randomly divided into saline group(n=8),magnesium chloride group(n=8)and minocycline group(n=8).The closed spinal cord injury model was prepared with balloon compression in the dorsal spinal cord of rat,which was evaluated with magnetic resonance imaging.All rats were successively administered their own drugs for seven days after injury,respectively.They were assessed with BBB score two to 31 days after operation.Their motor-evoked potential and sensory-evoked potential were detected 31 days after operation,and then Luxol Fast Blue was used to observe the area of secondary injury.Results Animal magnetic resonance imaging showed hypointense in T 2 images in T 10 spinal cord.BBB score was more in the minocycline group than in the saline group since 17 days after operation(P<0.05).The amplitude of motor evoked potential was higher in the minocycline group than in the saline group(P<0.05),while the area of secondary injury was less(P<0.05).Conclusion Minocycline may protect the nerves from secondary injury after acute spinal cord injury.