基于GC-MS研究不同产地三色藜麦种子的代谢物差异

Study on the metabolite difference of tricolor quinoa from different habitats based on GC-MS

为了研究不同生境对藜麦发育的影响,以不同产地的藜麦种子为研究对象,筛选显著差异代谢物质和相关代谢通路,利用GC-MS平台进行非靶向代谢组学分析,分析了青海都兰县和四川盐源县两地产出的黑藜、红藜、白藜种子的代谢轮廓,将原始数据经XCMS online平台预处理后进行后续多元统计分析。结果表明,青海都兰县和四川盐源县两地的黑、红、白藜麦种子的代谢特征均不相同,其中红藜麦种子与白藜麦种子差异较大,而黑藜麦种子差异最小;红藜存在显著差异,共有特征峰570个,上调物少于下调物;黑藜差异最小,上、下调趋势与红藜一致;白藜差异较大,上、下调趋势与红藜和黑藜相反,共有特征峰486个,上调物质多于下调物质;不同生境对藜麦的影响较大,黑藜和红藜更适宜在青海都兰县种植,而白藜更适宜在四川盐源县种植。研究结果可以指导青海都兰县和四川盐源县两地藜麦的栽培育种,也为深入研究藜麦的抗逆性机理提供了理论依据。

In order to study the effects of different habitats on the development of quinoa, taking quinoa seeds from different habitats as research objects, significant differences in metabolites and related metabolic pathways are screened. The metabolic profiles of the seeds of black quinoa, red quinoa and white quinoa from Dulan County, Qinghai Province and Yanyuan County, Sichuan Province are analyzed through non-targeted metabonomics analysis with GC-MS platform. The original data are pretreated by XCMS online platform for subsequent multivariate statistical analysis. The results show that the metabolic characteristics of black, red and white seeds in Dulan county and Yanyuan County are different. Among them, the difference between red and white seeds from the two counties is larger, while the difference of the black quinoa seeds is the smallest. There are 570 characteristic peaks in red seeds which have significant differences, and the up-regulated substances are less than the down-regulated. The difference between black quinoa from two habitats is the smallest, and the up-and down-regulated trends are like that of red quinoa. There is secondly significant difference between the white seeds from the two habitats, and the up-and down-regulated trends are the opposite of that of the red and black quinoas, and 486 characteristic peaks are detected, with more up-regulated substances than down-regulated. Different habitats have great impact on quinoa. Black and red quinoas are more suitable to be planted in Dulan County, Qinghai Province, while white quinoa is more suitable to be planted in Yanyuan County, Sichuan Province. The conclusions could guide the cultivation and breeding of quinoa in Dulan county and Yanyuan County, and provide theoretical basis for further study of the stress resistance mechanism of quinoa.