北京市人群尿液中羟基多环芳烃的影响因素

Study on polycyclic aromatic hydrocarbons metabolites in urine of typical population in Beijing and the influencing factors

于2016年11、12月以北京市3个典型区域的431名普通居民为研究对象,平均年龄(62.80±10.42)岁.以液相色谱联合质谱检测人群尿液中2-OHNap、1-OHNap、2-OHFul、1+9-OHPhe、2-OHPhe、3-OHPhe、4-OHPhe及1-OHPyr浓度水平,并对研究人群进行问卷调查.结果表明,全部人群尿液中2-OHNap、1-OHNap、2-OHFul、∑OHPhe、1-OHPyr浓度中位数水平分别为2.99,3.46,4.24,1.49,0.35μg/g Cr.Logistics回归分析显示,吸烟者尿液中2-OHNap、1-OHNap、2-OHFul、1-OHPyr发生高浓度的可能性分别是不吸烟者的9.83,6.32,4.51,1.89倍;年龄组每增加一个等级,导致2-OHNap、1-OHNap、∑OHPhe、1-OHPyr发生高浓度的可能性分别增加了0.48,0.44,0.31,0.46倍;教育程度每增加一个等级,导致2-OHFul发生高浓度的可能性降低了0.44倍.相关性分析显示,人体尿液中2-OHNap的浓度与环境空气中的萘的浓度呈正相关关系.人体尿液中2-OHNap主要来源于城市空气中的萘.影响北京市典型区域人群尿样中羟基多环芳烃浓度升高的主要因素为吸烟、年龄增高以及受教育程度较低.

The aim of this study is to investigate the levels of polycyclicaromatic hydrocarbons (PAHs) metabolites in the urine collected from a representative resident sample in Beijing and the relevant influencing factors. The resident sample consisted of 431 ordinary residents (average age (62.80±10.42)) recruited in three typical regions of Beijing in November and December 2016. The concentrations of 2-OHNap, 1-OHNap, 2-OHFul 1+9-OHPhe, 2-OHPhe, 3-OHPhe, 4-OHPhe and 1-OHPyr in the urine samples were studied by high performance liquid chromatography with mass spectrometry (LC-MS). Information of the subjects was gathered by a unified questionnaire. The median concentration of 2-OHNap, 1-OHNap, 2-OHFul,∑OHPhe and 1-OHPyr in the urine samples was 2.99, 3.46, 4.24, 1.49, 0.35μg/g Cr, respectively. Logistics regression analysis showed that the probability of high concentration of 2-OHNap, 1-OHNap, 2-OHFul and 1-OHPyr for smokers was 9.83, 6.32, 4.51 and 1.89 times higher than that of non-smokers, respectively. The probability of high concentration of 2-OHNap, 1-OHNap,∑OHPhe and 1-OHPyr was shown to increase by 0.48, 0.44, 0.31 and 0.46 times with each increased age level, respectively. The probability of high concentration of 2-OHFul was decreased by 0.44 times with each increased level in education attainment. The study indicated that 2-OHNap, 2-OHFul and ∑OHPhe in human urine were mainly derived from naphthalene, fluorene and phenanthrene in urban air. And the major factors influencing the increase of the concentrations of PAHs’ metabolites in human urine were smoking, age and relatively low educational level.