摘要
基于羧基量子点的荧光猝灭效应,提出了一种检测神经生长因子(NGF)的新方法。研究结果表明,在pH7.5的缓冲溶液中,NGF抗原与羧基量子点标记的NGF抗体发生特异性结合,能使羧基量子点的荧光发生猝灭。在1～20ng·mL^(-1)范围内,荧光猝灭程度与NGF抗原质量浓度呈良好的线性关系,线性系数为0.9696,检测下限为1ng·mL^(-1)。所提检测方法具有良好的抗干扰特性,在诊断检测方面具有巨大的应用潜力。
A new method for detecting the nerve growth factors (NGF) is proposed based on the fluorescence quenching effect of carboxyl quantum dots. The research results show that in the pH7.5 buffer solution, the NGF antigen specifically binds to the carboxyl quantum dot-labeled NGF antibody, which can quench the fluorescence of the carboxyl quantum dots. The fluorescence quenching degree and the NGF antigen mass concentration has a good linear relationship in the range of 1-20 ng/mL, and the linear correlation coefficient is 0.9696 with the detection limit of 1 ng/mL. This proposed method has good anti-interference characteristics and has great potential applications in diagnosis and detection.
作者
彭嫚
白忠臣
张莹
黎显继
张正平
Peng Man;Bai Zhongchen;Zhang Ying;Li Xianji;Zhang Zhengping(College of Big Data and Information Engineering,Guizhou University,Guiyang,Guizhou 550025,China;Key Laboratory for Photoelectronics and Application Technology,Guizhou University,Guiyang,Guizhou 550025,China;School of Mechanical & Electrical Engineering,Guizhou,Normal University,Guiyang,Guizhou 550025,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2019年第6期199-205,共7页
Laser & Optoelectronics Progress
基金
国家自然科学基金(11204046)
国家国际科技合作专项项目(2014DFA00670)
中央引导地方科技发展专项(QKZYD[2017]4004)
贵州省科技支撑项目(QKHZ[2017]2887)
关键词
激光技术
羧基量子点
荧光猝灭法
神经生长因子
抗原
抗体
laser technique
carboxyl quantum dots
fluorescence quenching method
nerve growth factor
antigen
antibody
