苹果糖转运蛋白基因MdSWEET1在番茄中异源表达提高其耐盐性

Ectopic Expressing MdSWEET1 in Tomato Enhanced Salt Tolerance

克隆得到苹果糖转运蛋白SWEET(sugars will eventually be exported transporters)基因MdSWEET1(MDP0000237435),组织表达分析发现该基因主要在苹果的茎和花中表达,相对定量分析发现其对NaCl、PEG、H_2O_2和ABA等均有响应。在番茄中异位表达MdSWEET1可提高植株耐盐性,并且可溶性糖含量,特别是蔗糖和果糖含量提高。

Sugars are important energy source and signaling molecule in plant,and play important roles in plant growth and development as well as biotic and abiotic stresses. Sugar cannot be transported independently across the plant biofilm system and needs the assistance of corresponding sugar transporters. SWEET(sugars will eventually be exported transporters)is a class of sugar transporters,it can carry out bidirectional transport of sugar in plants,and participate in many important physiological processes of plant growth and development by regulating the transport,distribution and storage of sugar compounds in plants. In this study,we cloned a gene named MdSWEET1. Expression analysis suggested that MdSWEET1 expressed mainly in stems and flowers and can respond to various abiotic stresses including NaCl、PEG、H2 O2 and ABA. Ectopic expressing MdSWEET1 in tomato enhanced salt tolerance and accumulated more soluble sugar especially sucrose and fructose.