细胞角蛋白18在HBV相关性肝病中的表达及临床意义

The expression and clinical significance of cytokeratin 18 in HBV associated liver disease

目的探讨细胞角蛋白18(CK18)在乙型肝炎病毒(HBV)相关性肝病中的表达及临床意义。方法选取2016年4月至2018年3月在该院诊断为无肝炎活动的慢性HBV感染者30例为未活动组,肝炎活动的HBV感染者28例为活动组,慢性乙型肝炎后肝硬化患者36例为肝硬化组,慢性乙型肝炎后肝硬化合并肝细胞癌患者32例为肝癌组,同时选取健康体检者20例作为健康对照组,检测入选者外周血清中细胞CK18表达水平。结果健康对照组、未活动肝炎组、活动肝炎组、肝硬化组、肝癌组患者外周血清中CK18表达水平分别为(112.133±3.443)ng/mL、(123.233±3.250)ng/mL、(148.467±0.379)ng/mL、(169.067±1.595)ng/mL、(185.167±1.595)ng/mL,健康对照组与未活动肝炎组两组间比较,差异无统计学意义(P>0.05);而活动性肝炎组与健康对照组相比,肝硬化组与活动性肝炎组相比,肝癌组与肝硬化组相比,差异均有统计学意义(P<0.05)。结论 CK18表达水平与HBV相关性肝病发生、发展有关,可作为预测HBV相关性肝病严重程度的指标之一。

Objective To investigate the expression and clinical significance of cytokeratin 18 in HBV-associated liver disease.Methods From Apr 2016 to Mar 2018,collect 30 patients with hepatitis-free activities chronic hepatitis B virus infection were selected as unactive hepatitis group,28 patients with hepatitis activities chronic hepatitis B virus infection were selected as activities hepatitis group,36 patients with cirrhosis after chronic hepatitis B were selected as cirrhosis group,32 patients with liver cirrhosis and hepatocellular carcinoma after chronic hepatitis B were selected as liver cancer group,and 20 healthy health examiners were selected as healthy controls,CK18 expression levels of peripheral serum cells of all the samples were detected.All the people were selected from affiliated hospital of hebei university of engineering.Results The expression of CK18 in the healthy control group,unactive hepatitis group,active hepatitis group,liver cirrhosis group and liver cancer group were (112.133±3.443)ng/mL,( 123.233± 3.250)ng/mL,(148.467±0.379)ng/mL,( 169.067± 1.595)ng/mL and (185.167±1.595)ng/mL respectively,there was no difference between the unactive hepatitis group and the healthy control group ( P <0.05);while between the active hepatitis group and the healthy control,liver cirrhosis group and active hepatitis group,liver cancer group and liver cirrhosis group,the difference were all have statistically significant ( P <0.05).Conclusion The expression level of CK18 was related to the development of HBV-associated liver disease,and may be one of the indicators for predicting the severity of HBV-related liver disease.