长链非编码RNA HIT在肝癌中的表达及意义

Expression and Significance of Long Non-coding RNA HIT in the Hepatocellular Carcinoma

目的:探讨长链非编码RNA HIT(lncRNA-HIT)在原发性肝癌中的表达及其与患者临床病理特征的相关性,寻找肝癌治疗的新靶点。方法:收集并筛选2015年1月至2018年1月在空军军医大学第一附属医院行手术治疗的80例经病理证实的肝细胞癌患者的肝癌组织和癌旁组织。采用实时定量聚合酶连反应(q RT-PCR)法检测患者手术切除的肝癌组织及相应的癌旁组织中lncRNA-HIT的表达,并分析HIT与患者临床病理参数之间的关系。结果:原发性肝癌组织中lncRNA-HIT的表达(6.17±4.38)明显高于癌旁组织(2.81±2.58),约为癌旁组织的2.20倍(P<0.05),肝癌组织中HIT的表达与肝癌TNM分期、肿瘤大小和数量显著相关(P<0.05),而与性别、年龄、肝硬化、HBV、AFP无显著相关性(P>0.05)。结论:lncRNA-HIT在肝癌组织中呈高表达,可能在肝癌发生发展过程起重要作用,并可能作为肝癌治疗的新靶点。

Objective: To explore the expression of lncRNA HIT in the hepatocellular carcinoma (HCC) and its correlation with the clinicopathologic characteristics of patients, and find new target for HCC treatment. Methods: From January 2015 to January 2018, the liver cancer tissues and adjacent tissues of 80 cases of patients who underwent surgical treatment were collected and screened with pathologically confirmed hepatocellular carcinoma in the First Affiliated Hospital of Air Force Military Medical University. qRT-PCR was performed to measure the expression level of the lncRNA HIT in 80 cases of HCC specimens and paired adjacent normal liver tissues (ANLTs) after surgery and the correlation between lncRNA HIT expression in the HCC patients and clinical characteristics were further analyzed. Results: The expression of lncRNA HIT in HCC tissue (6.17± 4.38) was significantly higher than that in the ANLTs (2.81± 2.58), nearly 2.20 times to ANLTs (P<0.05). The expression of lncRNA HIT in HCC tissues was significantly related with the TNM stage, tumor size and number. However, there was no obvious correlation with the age, gender, liver cirrhosis, HBV and AFP (P> 0.05). Conclusion: The expression of lncRNA HIT was up-regulated in the HCC tissues, it may be involved in in the development and progression of HCC and used as a new target for the treatment of HCC.