摘要
为了改善猪肉品质,探究肌肉抑制素(MSTN)通过硬脂酰辅酶A去饱和酶5(SCD5)调控脂质代谢的分子通路,试验采用qPCR和Western-blot方法检测MSTN野生型细胞系PK15、单等位基因敲除型细胞系PK3108及双等位基因敲除型细胞系L18中SCD5的表达量变化。结果表明:在mRNA水平,MSTN单等位基因和双等位基因敲除引起SCD5 98%和96%的下调;在蛋白质水平,MSTN单等位基因敲除对SCD5影响较小,双等位基因敲除引起SCD5大幅下调。说明在细胞水平上,失活MSTN可引起SCD5表达量下调。
In order to improve the meat quality of pork and explore the molecular pathway of MSTN regulating lipid metabolism by stearoyl-Co A desaturase 5 ( SCD5), SCD5 expression level of MSTN wild-type cell line PK15, single allele knockout cell line PK3108 and MSTN biallelic gene knockdown cell line L18 were detected by using qPCR and Western-blot. The results showed that at mRNA level, MSTN single allele and biallelic knockout caused 98% and 96% down-regulation of SCD5. At protein level, the MSTN single allele knockout had little effect on SCD5 while the biallelic knockout caused significant down-regulation of SCD5. It proves that inactivation of MSTN causes down-regulation of SCD5 at cellular level.
作者
蔡刚志
华再东
毕延震
陈彬
CAI Gangzhi;HUA Zaidong;BI Yanzhen;CHEN Bin(Key Laboratory of Plateau Mountain Animal Genetics,Breeding and Reproduction,Ministry of Education,Guizhou University,Guiyang 550025,China;Key Laboratory of Animal Genetics,Breeding and Reproduction in Guizhou,Guiyang 550025,China;College of Animal Science,Guizhou University,Guiyang 550025,China;Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Science/Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Wuhan 430064,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2019年第6期29-31,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31772577)
湖北省自然科学基金"杰出青年"项目(2018CFA043)
湖北省农业科技创新中心项目(2018-620-004-001)
湖北省农业科学院领军人才培养计划项目
