柯萨奇病毒A16型手足口病与IL-1β、IL-1RN及TNF-α单核苷酸多态性的相关性

Correlation Between Single Nucleotide Polymorphism of IL-1β,IL-1RN and TNF-α and Hand,Foot and Mouth Disease Caused by Coxsackievirus A16

目的探讨炎性细胞因子白介素(IL)-1β、IL-1RN及TNF-α单核苷酸多态性与柯萨奇病毒A16型(CA16)感染手足口病(HFMD)的相关性。方法以西安交通大学第二附属医院和西安市儿童医院感染科收住的83例CA16 HFMD患儿及健康儿童201例为研究对象,采用SNPscan^(TM)多重SNP技术检测上述样本IL-1βrs16944、rs1143627,IL-1RN rs315952、rs9005及TNF-αrs1800629、rs1799964位点的基因分型。结果 284例CA16感染患儿及健康对照儿童,IL-1RN rs315952 C等位基因的患病风险升高(C vs.T:OR=1.268,95%CI:1.043～1.542),IL-1RN rs9005 A等位基因患病风险降低(A vs.G:OR=0.763,95%CI:0.616～0.946),而IL-1βrs16944、rs1143627及TNF-αrs1800629、rs1799964的单核苷酸多态性与CA16 HFMD患病风险无明显相关性。结论 IL-1RN rs315952、rs9005单核苷酸多态性与CA16型HFMD患病风险相关联。

Objective To investigate the correlation between single nucleotide polymorphism of interleukin (IL)-1β,IL-1RN,TNF-α and hand-foot-mouth disease (HFMD) caused by coxsackievirus A16 (CA16) infection.Methods A total of 83 children with CA16 HFMD and 201 healthy children were enrolled. The SNPscan multiplex SNP technique was used to detect the genotyping of IL-1β rs16944,rs1143627,IL-1RN rs315952,rs9005 and TNF-α rs1800629,rs1799964 loci. ResultsThe susceptibility risk of IL-1RN rs315952 allele C was significantly increased (C vs. T:OR=1.268,95% CI:1.043~1.542),while it was reduced in IL-1RN rs9005 allele A (A vs. G:OR=0.763,95% CI:0.616~0.946). No association of gene polymorphism with susceptibility risk of CA16 HFMD was observed in IL-1β rs16944,rs1143627 and TNF-α rs1800629,rs1799964. ConclusionThe study indicates that the gene polymorphism of IL-1RN rs315952 and rs9005 is associates with susceptibility risk of CA16 HFMD.