狼疮肾炎患者肾组织巨噬细胞浸润与骨桥蛋白表达的关系

The relationship between osteopontin and macrophage infiltration in renal tissues of patients with lupus nephritis

目的探讨LN中巨噬细胞在肾脏组织局部浸润与骨桥蛋白表达的关系及LN发病的潜在机制。方法选择初次诊治经肾活检病理检查确诊为LN的患者60例及10例因肾脏外伤行肾脏切除患者的正常肾脏组织作为对照组。通过免疫组织化学染色方法检测60例LN患者及10例正常对照组患者的肾组织中骨桥蛋白的表达和巨噬细胞(CD68+细胞)的浸润。采用方差分析,LSD-t检验,以及Pearson相关分析。结果①与正常对照组(5.3±1.8)相比,LN患者肾组织中浸润的巨噬细胞的数量显著增加,并且活动组LN患者肾内浸润的巨噬细胞数量多于非活动组[(52.3±7.3)和(26.1±7.2)](F=105.20,P<0.05)。②与正常对照组(13.3±2.9)相比,LN患者的骨桥蛋白(23.5±4.5)表达也明显增加,活动组患者骨桥蛋白(21.5±3.7)的表达水平显着高于非活动组(27.0±2.4)(F=28.65,P<0.05)。③本次检测的LN患者肾组织中,巨噬细胞浸润与骨桥蛋白表达呈正相关(r=0.610,P<0.01)。结论骨桥蛋白的高表达是LN发病过程中巨噬细胞在肾脏组织局部浸润的重要介导因子,可能是LN发病的潜在机制之一。

Objective To investigate the relationship between the expression of osteopontin (OPN) and the infiltration of macrophages in renal tissues of patients with lupus nephritis (LN), and provide the evidence for the pathogenesis of lupus nephritis. Methods The expression of OPN and the infiltration of macrophages (CD68+ cells) in the renal tissue of 60 LN patients and 10 normal controls were detected by immunohistoche-mical method. Statistical analysis was used to analyze the correlation between variables. Results ① Compared with the normal control group (5.3±1.8), the number of macrophages infiltrated inthe renal tissue of LN patients was significantly increased [(52.3±7.3) vs (26.1±7.2), F=105.20, P<0.05].② Compared with the normal control group, the expression of OPN in LN patients was significantly increased, and the expression level of OPN in active patients was significantly higher than that in the inactive group [(23.5±4.5) vs (13.3±2.9),(21.5±3.7) vs (27.0±2.4), F=28.65, P<0.05].③ There was a significant positive correlation between macrophage infiltration and renal OPN expres-sion in renal tissue of LN patients (r=0.610, P<0.01). Conclusion The high expression of OPN is an im-portant mediator of macrophage infiltration in the process of lupus nephritis development, which may be one of the potential mechanisms of lupus nephritis.