慢性酒精中毒大鼠在学习记忆方面差异基因的生物信息学分析

Bioinformatics Analysis of Differential Genes Related to Learning and Memory in Chronic Alcoholism in Rats

借助基因芯片获取慢性酒精中毒大鼠海马相关基因的表达数据集,通过生物信息学的分析方法对差异表达基因进行筛选与分析。从分子水平揭示慢性酒精中毒对大鼠大脑海马体的影响,为慢性酒精中毒的损伤机制以及相关疾病发病机制的基础研究与临床治疗提供新的方向。同时,还通过Y迷宫实验对实验大鼠的学习记忆功能进行了检测,借助电镜拍摄其线粒体。结果显示,我们一共筛选出208个差异表达基因,其中51个表达上调,157个表达下调。其中涉及的主要信号通路有氧化磷酸化通路、D-谷氨酰胺和谷氨酸代谢通路、阿尔茨海默病信号通路、帕金森病信号通路、膀胱癌信号通路、B细胞受体信号通路和亨廷顿病信号通路等。由此我们得出结论,慢性酒精中毒可能影响了海马多个基因的表达,其中包括Rpsa、Wdr31、Rps11、Rps9、Ndufa2、Mrto4、Rpl6、Dap3、Ndufb8、Ndufb6、Ephb2、Cox6c、Prkcd、Rela、Raf1、Ubd、Mrps28、Mrpl35等关键基因,进而损伤了电子传递链复合体Ⅰ,最终损伤线粒体,导致大鼠学习记忆能力的损伤。

Gene expression data sets of hippocampus in chronic alcoholism rats were obtained by gene chip technology,and then we analyzed and screened the differentially expressed genes by bioinformatics analysis method.The study revealed the effects of chronic alcoholism on the hippocampus of rats,and provided a new direction for the basic study of the injury mechanism of chronic alcoholism and the pathogenesis of related diseases and their clinical treatment.In addition,the Y labyrinth experiment was used to detect the learning and memory function of the experimental rats,and the mitochondria were photographed by electron microscope.A total of 208 differentially expressed genes were screened out,51 of which were up-regulated and 157 were down regulated.The main pathways involved included the oxidative phosphorylation pathway,D-glutamine and glutamate metabolism pathway,signal pathways of Alzheimer's disease,Parkinson's disease,bladder cancer,B cell receptor,and Huntington's disease,In conclusion,chronic alcoholism may affect the expression of multiple key genes in hippocampus of rats,including Rpsa,Wdr31,Rps11,Rps9,Ndufa2,Mrto4,Rpl6,Dap3,Ndufb8,Ndufb6,Ephb2,Cox6c,Prkcd,Rela,Raf1,Ubd,Mrps28,Mrpl35,which damage the electron transfer chain complex Ⅰ,and eventually damage the mitochondria,resulting in the impairment of learning and memory ability of rats.