基于转录组测序的云南‘火把梨’花青素相关基因分析

Anthocyanidin Related Gene Analysis of 'Huoba Pear' in Yunnan Based on Transcriptome Sequencing

本研究以云南‘火把梨’转色前后的梨果皮为试验材料,采用高通量测序技术(Illumina HiSeq TM 2000/MiSeq)对两种梨材料全转录功能基因组测序后组装,再通过GO、Swiss-Prot两大数据库进行花青素相关差异表达基因筛选,以了解可能与花青素合成相关的基因及其表达模式,同时将差异基因在NCBI数据库进行Blast序列比对,对其功能进行分析。最终共获得7.52 Gb数据,组装过滤后共获得75 227 538条Clean reads,3 920条差异表达基因,通过通路分析和Pathway功能注释,在花青素合成过程中的两条通路中共注释到30条与花青素合成相关的差异表达基因,初步对这些差异基因的功能进行鉴定和分析,对后期基因克隆及转基因等研究具有重要意义。

In this research, the peels of Yunnan ’Huoba Pear’ before and after color transformation was used as experimental materials, the full transcription functional genomes of the two pear materials were sequenced and assembled using high-throughput sequencing technology(Illumina HiSeqTM2000/MiSeq), and then anthocyaninrelated differentially expressed genes were screened through GO and Swiss-Prot databases to understand the genes and their expression patterns that might be associated with anthocyanin synthesis. At the same time, the Blast sequences of differential genes were compared in the NCBI database and their functions were analyzed. A total of7.52 Gb data was finally obtained. After assembly and filtration, 75 227 538 Clean reads and 3 920 differentially expressed genes were obtained. Through pathway analysis and Pathway function annotation, 30 differentially expressed genes related to anthocyanin synthesis were annotated in the two pathways during anthocyanin synthesis. The preliminary identification and analysis of the functions of these differential genes might be of great significance to the further gene cloning and transgenic resrarch work.