粉防己碱对激素非依赖性人前列腺癌细胞株PC-3增殖和凋亡影响的研究

The effect of tetrandrine on proliferation and apoptosis of human androgen refractory prostate cancer PC-3 cell line

目的探讨粉防己碱(TET)对激素非依赖性人前列腺癌细胞株PC-3细胞增殖抑制和凋亡作用及其机制。方法应用CCK法观察不同浓度TET(1、2、4、8μg/mL)对人前列腺癌细胞株PC-3生长的抑制作用;用Annexin-V与PI双染法流式细胞术分析TET对PC-3细胞凋亡的影响;RT-PCR检测TET对人前列腺癌细胞株PC-3IL-8表达水平的影响。结果TET对前列腺癌PC-3细胞株增殖有抑制作用,其抑制效应呈剂量依赖性。不同浓度TET作用48h后,前列腺癌PC-3细胞株的凋亡率随着浓度的增高而升高,差异具有统计学意义(P<0.01)。不同浓度TET作用48h后IL-8mRNA表达水平随着浓度的增加而降低,差异具有统计学意义(P<0.01)。结论TET能抑制人前列腺癌细胞株PC-3细胞增殖,并促进人前列腺癌细胞株PC-3的细胞凋亡,其作用机制可能与其下调IL-8mRNA表达有关。

Objective To investigate the mechanism of proliferation and apoptosis of human androgen-refractory prostate cancer PC-3 cell line by tetrandrine.Methods The effects of TET(1μg/mL,2μg/ml,4μg/mL,8μg/mL)on the growth of PC-3 cell line were measured by CCK assay.The apoptosis of cell line induced by TET(1μg/mL,2μg/mL,4μg/mL,8μg/mL)was evaluated by Annexin-V and PI followed by flow cytometry(FCM).The effects of TET on the expression of IL-8 were measured by RT-PCR.Results TET inhibited the proliferation of PC-3 cell line in a dose dependent manner.After treated with TET(1μg/mL,2μg/mL,4μg/mL,8μg/mL)for 48 h,the apoptotic rate increased with the increase of drug concentration(P<0.01).Similarly,the expression of IL-8 mRNA decreased gradually with the increase of drug concentration(P<0.01).Conclusions TET can inhibt the proliferation of PC-3 cell line,and induce apoptosis.This may be associated with the decreasing expression of IL-8 mRNA.