藤黄酸提取分离及其抗肿瘤活性

Extraction and separation of gambogic acid and its antitumor activity

[目的]优化中药藤黄中总藤黄酸的提取分离方法,对藤黄酸抗肿瘤活性进行初步研究。[方法]利用回流法进行提取,通过单因素考察实验,研究不同因素对总藤黄酸提取率的影响,并通过正交实验对提取工艺进行优化。再将总藤黄酸粗品进一步分离纯化得到藤黄酸,并进行表征;采用四氮唑蓝(MTT)比色法考察藤黄酸对两种癌细胞的体外抗肿瘤活性。[结果]总藤黄酸最佳的提取工艺为:提取溶剂为乙酸乙酯,料液比为1∶6(g/m L),提取次数为2次,提取时间为3 h,提取温度为80℃,提取率约为57.0%。通过表征确定纯化后所得是藤黄酸。且藤黄酸对癌细胞具有一定的抑制作用。[结论]正交试验优选的总藤黄酸提取工艺简便、稳定、可行,藤黄酸有较强的抗癌作用。

[Objective]To optimize the extraction and separation method of total gambogic acid from Chinese medicine Garcinia Cambogia,and to study the antitumor activity of gambogic acid.[Methods]The extraction process was carried out by reflux method.The effects of different factors on the extraction rate of total gambogic acid were studied by single factor investigation.The extraction process was optimized by orthogonal experiment.The crude total gambogic acid is further separated and purified to obtain gambogic acid,and characterized.The in vitro antitumor activity of gambogic acid against two cancer cells was investigated by MTT colorimetry.[Results]The best extraction process of total gambogic acid was as follows: the extraction solvent is ethyl acetate,the ratio of material to liquid is 1∶6(g/m L),the extraction times are 2,the extraction period is 3 hours,the extraction temperature is 80 ℃,and the extraction rate is 57.0 %.After purification,the obtained product was confirmed by characterization to be gambogic acid,which demonstrated a certain inhibitory effect on cancer cells.[Conclusion]The optimal extraction process of total gambogic acid from orthogonal test is simple,stable and feasible,and gambogic acid has strong anticancer effect.