氧化苦参碱对人脐静脉内皮细胞体外缺血再灌注损伤的保护作用及其分子机制

Protective Effect of Oxymatrine on Human Umbilical Vein Endothelial Cells Ischemia-reperfusion Injury in Vitro and Its Molecular Mechanism

目的研究氧化苦参碱(oxymatrine,OMT)对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)体外缺血再灌注(ischemia reperfusion,I/R)损伤的保护作用及其分子机制。方法将HUVEC分为对照组、I/R组和OMT 0. 5、1、2μg/ml组,对照组为正常培养细胞,I/R组和OMT 0. 5、1、2μg/ml组均制备I/R损伤模型,OMT0. 5、1、2μg/ml组分别给予OMT 0. 5、1、2μg/ml处理。检测5组细胞存活率、乳酸脱氢酶(LDH)含量、细胞凋亡情况及Bcl-2、Bax、Caspase-3蛋白表达水平。结果与I/R组比较,OMT 0. 5、1、2μg/ml组HUVEC存活率升高,培养液中LDH含量降低,细胞凋亡减少,Bcl-2/Bax逐渐上调,Caspase-3蛋白表达下调,且呈剂量依赖性(P <0. 05,P <0. 01)。结论 OMT对HUVEC体外I/R损伤有保护作用,其机制可能与减少细胞凋亡有关。

Objective To study protective effect of Oxymatrine (OMT) on human umbilical vein endothelial cells (HUVEC) ischemia-reperfusion (I/R) injury in vitro and its molecular mechanism. Methods HUVEC were divided into control group, I/R group, 0.5, 1 and 2μg /ml OMT groups. Control group was normal cultured cells. I/R group, 0.5, 1 and 2μg/ml OMT groups were prepared I/R injury models. The 0.5, 1 and 2μg/ml OMT groups were treated with 0.5, 1 and 2μg/ml OMT respectively. Cell survival rate, lactate dehydrogenase (LDH) contents, conditions of cell apoptosis and expressions of Bcl-2, Bax and Caspase-3 proteins were detected among 5 groups. Results Compared with those in I/R group, in 0.5, 1 and 2μg /ml OMT groups, survival rates of HUVEC were increased;LDH contents in culture medium were decreased;numbers of cell apoptosis were decreased;values of Bcl-2/Bax were increased gradually;expressions of Caspase-3 proteins were decreased in a dose-dependent manner ( P <0.05, P <0.01). Conclusion Oxymatrine has protective effects on HUVEC ischemia-reperfusion injury in vitro, and its mechanism may be related to reduction of apoptosis.