摘要
目的分析微RNA-133a(miR-133a)和微RNA-539(miR-539)在骨肉瘤组织中的表达情况,并探讨miR-133a和miR-539在骨肉瘤组织中的临床病理特征及与预后的相关性。方法收集2011年6月—2013年6月手术切除的骨肉瘤组织标本45例和瘤旁正常组织标本45例,分析患者临床资料、骨肉瘤组织中miR-133a和miR-539的表达与患者临床病理特征和预后的关系。结果骨肉瘤组织中miR-133a和miR-539水平低表达率高于瘤旁正常组织(P <0. 05)。临床分期Ⅲ~Ⅳ期、中+低分化、T3~T4期及miR-133a、miR-539水平低表达均为影响骨肉瘤患者预后的独立危险因素(P <0. 05)。结论 miR-133a、miR-539水平在骨肉瘤组织中以低水平表达,其与临床分期为Ⅲ~Ⅳ期、中+低分化、T分期为T3~T4期共同为影响骨肉瘤患者预后的独立危险因素。
Objective To analyze expressions of microRNA-133a (miR-133a) and microRNA-539 (miR-539) in osteosarcoma tissues and to investigate correlations with clinicopathological features of miR-133a and miR-539 in osteosarcoma tissues and prognosis. Methods A total of 45 osteosarcoma tissue specimens and 45 adjacent normal tissue specimens by surgical ablation during June 2011 and June 2013 were collected. Clinical data of patients, expressions of miR-133a and miR-539 in osteosarcoma tissues and their relationship with clinicopathological features and prognosis were analyzed. Results Low expression rates of miR-133a and miR-539 in osteosarcoma tissues were higher than those in adjacent normal tissues ( P <0.05). Clinical stage III-IV, moderate+lower differentiation, stage T3-T4 and low expressions of miR-133a and miR-539 were independent risk factors for prognosis of patients with osteosarcoma ( P <0.05). Conclusion The miR-133a and miR-539 show low expressions in osteosarcoma tissues, and the low expressions, clinical stage III-V, moderate+lower differentiation and stage T3-T4 are all independent risk factors for prognosis of patients with osteosarcoma.
作者
吴志明
刘江涛
徐俊昌
黄彬
WU Zhi-ming;LIU Jiang-tao;XU Jun-chang;HUNAG Bin(Department of Orthopedics, the First People's Hospital of Xiangyang, Xiangyang, Hubei 441000, China)
出处
《解放军医药杂志》
CAS
2019年第4期32-36,共5页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
湖北省自然科学基金(2015CFA076)