心痛泰干预心肌缺血大鼠心肌中Notch1、Dll4蛋白表达的研究

Effect of Xintongtai Granule(心痛泰) on Notch1, Dll4 Protein Expression in Rats with Acute Myocardial Ischemia

目的:通过构建急性心肌缺血模型,探讨心痛泰对急性心肌缺血大鼠新生血管密度、缺血心肌中Notch1、Dll4蛋白表达的影响。方法:100只SD大鼠,除假手术组10只外,其余大鼠予以结扎大鼠冠状动脉左前降支造成急性心肌缺血损伤模型,成模大鼠随机分为模型组,心痛泰低、中、高剂量组及麝香保心丸组。分别给以蒸馏水,心痛泰低、中、高剂量及麝香保心丸进行灌胃2周后,采用免疫组化法测新生微血管密度及缺血区心肌组织Notch1、Dll4蛋白的表达情况。结果:模型组大鼠的缺血心肌微血管密度、缺血心肌中的Notch1、Dll4蛋白表达均高于假手术组(P<0.05);心痛泰各剂量组及麝香保心丸组大鼠缺血心肌微血管密度、缺血心肌中的Notch1、Dll4蛋白表达均高于模型组(P<0.05)。结论:心痛泰具有保护缺血心肌的作用,这可能与其能激活Notch信号通路,上调Notch1、Dll4蛋白在缺血区的表达,促进血管新生有关。

Objective: By establishing the acute myocardial ischemia (AMI) model, to investigate the effects of Xintongtai granule on new blood vessels density, the expression of Notch1, D114 protein in rats with AMI. Methods: The rats were randomly divided into six groups, except for the 10 rats in sham -operation group, the other rats were ligatured left anterior of coronary artery to establish the rat models of AML Then randomly di-vided into the model group, high-, medium- and low-dose of Xintongtai groups, and Shexiang Baoxin pill (麝香保心丸)group, which were treated separately with distilled water, high-dose, medium-dose and low-dose of Xin-tongtai and Shexiang Baoxin pill by gavaged for 2 weeks, the new blood vessels density in myocardial tissue, the expression of Notch1 and D114 protein in myocardial tissue was tested using immunohistochemical method. Results: The expression of Notch1 and D114 in ischemic myocardium and the microvessel density of ischemic myocardium in model group were higher than those in sham-operated group (P<0.05), and the expression of Notch1 and D114 in ischemic myocardium in Xintongtai group and Shexiang Baoxin Pill group were higher than those in model group (P<0.05). Conclusion: Xintongtai has protective effect on ischemic myocardium, which may-be related to its activation of Notch signaling pathway, up-regulation of Notch1 and D114 protein expression in ischemic area and promotion of angiogenesis.