摘要
目的:采用高效液相-紫外检测器色谱法测定低聚木糖含量。方法:利用1-苯基-3-甲基-5-吡唑啉酮(PMP)进行衍生,采用高效液相-紫外检测器色谱仪测定低聚木糖含量。结果:色谱柱:ACE C18(4.6mm×250mm);流动相:乙腈-0.02mol/L乙酸铵溶液(17∶83);柱温:30℃;波长:250nm;流速:1.0mL/min;进样量:5μL。低聚木糖在0.02~0.2mg·mL^(-1)浓度范围内与峰面积积分呈良好的线性关系,回归方程为y=1.1281x+0.0123,R^2=0.9996。结论:建立的方法准确可靠,重复性好,分离度高,灵敏度强且操作简单,可用于测定低聚木糖的含量。
Objective:To establish Testing method and Methodological investigation of xylo-oligosaccharide by HPLC UV method.Methods:The content of xylo-oligosaccharide was determined by HPLC using derivatization of 1-phenyl-3-methyl-5-pyrazolinone(PMP).Results:Chromatographic conditions:chromatographic column:ACE C18(4.6 mm X 250 mm),Mobile phase:ammonium acetate solution of acetonitrile-0.02 mol/L(17:83),Column temperature:30℃;Wavelength:250nm,Flow rate:1.0 mL/min,Sampling quantity:5 μL.In the range of 0.02.0.2 mg.mL^-1,the peak area has a good linear relationship with the sample intake.The regression equation is y=l.1281x + 0.0123,R^2=0.9996.Conclusion:The method is accurate and reliable,and of good repeatability,high separating degree and sensitivity,and it is simple and easy to implement.It can be used to determine the content of oligosaccharide.
作者
高畅
姜文月
廉淑梅
邹慧
周立梅
杨磊
张金秋
刘念远
Gao Chang;Jiang Wenyue;Lian Shumei;Zou Hui;Zhou Limei;Yang Lei;Zhang Jinqiu;Liu Nianyuan(Jilin Modern Chinese Medicine Engineering And Research Center Co.,Ltd.,ChangChun 130000,China;Jilin Xiuyangtang Health Industry Co.,Ltd.,Fusong 134500,China)
出处
《亚太传统医药》
2019年第3期47-49,共3页
Asia-Pacific Traditional Medicine
基金
吉林省科技发展计划资助项目(20180311040YY)
关键词
低聚木糖
PMP衍生
高效液相-紫外检测器色谱法
Xylo-oligosaccharide
1-phenyl-3-methyl-5-pyrazolone(PMP)Derivatization
HPLC-UV Detector Chromatography