摘要
目的:利用ITS序列对不同来源辽细辛进行DNA分子鉴定。方法:提取辽细辛植物的DNA,对其ITS序列进行逆转录-聚合酶链式反应(RT-PCR)扩增与测序,且从GenBank中下载上述所属植物的ITS序列,进行辽细辛的比对。结果:从NJ系统发育树分析看出,两属4个物种的26个样本被聚为3大支。细辛植物自成1支,汉城细辛属1支,马兜铃属植物1支。结论:表明ITS序列可以对细辛原植物进行鉴别,且在近缘物种鉴定方面具有较大应用价值。
Objective:To characterize DNA molecules from different sources of Asarum by ITS sequences.Methods:The DNA of the plant was extracted and its ITS sequence was amplified and sequenced by reverse transcription-polymerase chain reaction(RTPCR).The ITS sequences of the above-mentioned plants were downloaded from GenBank.Comparison.Results:From the analysis of the NJ phylogenetic tree,26 samples of 2 genera and 4 species were clustered into three major branches.Asarum plants from one,Seoul Asarum is a branch.Aristolochia plant 1.Conclusion:It is shown that the ITS sequence can be used to identify the original plant,and it has great application value in the identification of the similar species.
作者
刘洋洋
赵容
梁一凡
殷楚璇
Liu Yangyang;Zhao Rong;Liang Yifan;Yin Chuxuan(Liaoning University of Traditonal Chinese Medicine,Dalian 116600,China)
出处
《亚太传统医药》
2019年第3期57-60,共4页
Asia-Pacific Traditional Medicine
基金
辽宁中医药大学大学生创新创业训练计划(201610162074)
关键词
辽细辛
ITS
分子鉴定
Liao Asarum
ITS
Molecular identification
