miR-509-3p及XIAP表达与肝癌细胞增殖侵袭能力影响

Effects of miR-509-3p and XIAP expression on proliferation and invasion of hepatocarcinoma cells

目的了解微小RNA(micro RNA,miR)miR-509-3p和XIAP表达对肝癌细胞增殖侵袭能力的影响及其作用机制。方法应用实时定量PCR(RT-PCR)检测107例肝癌患者肝癌组织和癌旁组织中miR-509-3p和XIAP表达,利用细胞增殖实验和Transwell侵袭实验观察转染miR-509-3p类似物和抑制剂的HepG2细胞、RNA干扰XIAP表达的HepG2细胞的增殖和侵袭能力。结果肝癌组织miR-509-3p与XIAP的相对表达量分别为0.415±0.098、1.657±0.147,癌旁组织miR-509-3p与XIAP的相对表达量分别为1.127±0.126、0.425±0.113,癌组织中miR-509-3p相对表达量显著低于癌旁组织,差异有统计学意义(t=3.257,P=0.002),而XIAP的相对表达量显著偏高(t=4.201,P=0.000)。肝癌组织miR-509-3p与XIAP mRNA表达水平呈负相关(r=0.218,P=0.046)。与对照组相比,转染miR-509-3p类似物48、72、96及120 h时,HepG2细胞增殖率明显偏低,差异有统计学意义(均P<0.05);与对照组相比,转染miR-509-3p抑制剂48 h后HepG2细胞增殖明显偏高(P均<0.05),与对照组相比,转染miR-509-3p类似物后24 h HepG2细胞侵袭数目明显较低,分别为96.32±0.52、51.47±0.45,差异有统计学意义(t=2.263,P=0.021);与对照组相比,转染miR-509-3p抑制剂后24 h HepG2细胞侵袭数目明显较高,分别为94.65±0.42、120.14±0.45,差异有统计学意义(t=2.463,P=0.013)。结论 miR-509-3p与XIAP的表达与肝癌细胞的增殖与侵袭能力有关,miR-509-3p表达可能通过影响XIAP表达而发挥调节肝癌细胞增殖和侵袭能力的作用。

Objective To investigate the influence of the expression of microRNA-509-3p(miR-509-3p)and X-linked inhibitor of apoptosis protein(XIAP)on the proliferation and invasion of hepatocarcinoma cells,and to explore the mechanism.Methods Real-time polymerase chain reaction was used to detect the expression of miR-509-3p and XIAP in 107 liver cancer patients’cancer tissues and paracancerous tissues.Cell proliferation assay and Transwell chamber invasion assay were used to observe the proliferation and invasion of HepG2 cells which were transfected with miR-509-3p mimics,miR-509-3p inhibitors,small interfering RNA silencing XIAP,respectively.Results The relative expression levels of miR-509-3p and XIAP in liver cancer tissues were 0.415±0.098 and 1.657±0.147,respectively,in paracancerous tissues were 1.127±0.126 and 0.425±0.113,respectively.The relative expression of miR-509-3p in liver cancer tissues was significantly lower than that in paracancerous tissues(t=3.257,P=0.002),while the relative expression of XIAP was significantly higher(t=4.201,P=0.000).There was a negative correlation between miR-509-3p and XIAP mRNA expression in liver cancer tissues(r=0.218,P=0.046).Compared with the control group,the proliferation rate of HepG2 cells was significantly lower at 48,72,96 and 120 hours of transfection with miR-509-3p analogs,and the difference was statistically significant(P<0.05).The proliferation rate of HepG2 cells was significantly higher after 48 hours of transfection with miR-509-3p inhibitors(P<0.05).Compared with the control group,the number of invasive HepG2 cells at 24 hours of transfection with miR-509-3p analogs was lower(96.32±0.52,51.47±0.45,t=2.263,P=0.021),the difference was statistically significant.Compared with the control group,the number of invasive HepG2 cell at 24 hours of transfection with miR-509-3p inhibitor was higher(94.65±0.42,120.14±0.45,t=2.463,P=0.013),the difference was statistically significant.Conclusion The expression of miR-509-3p and XIAP are related to the proliferation and invasion of hepatocarcinoma cells.MiR-509-3p may regulate the proliferation and invasion of hepatocarcinoma cells by affecting the expression of XIAP.