呼吸道合胞体病毒感染患儿鼻黏膜拭子样品微RNA表达

Expression of microRNAs in nasal mucosal swab samples from children with respiratory syncytial virus infection

目的研究呼吸道合胞体病毒(RSV)感染患儿鼻黏膜拭子样品微RNA(miRNA)表达及与症状轻重间的关系。方法选取2017年9月至2018年2月该院收治的26例确诊为RSV感染阳性患儿作为试验组,按临床症状分轻度17例,重度9例。选择同期来院儿保门诊就诊的12例健康儿童作为对照组。采集鼻黏膜上皮脱落细胞样品,通过miRNAs微阵列芯片分析其中2例重度、2例轻度和2例健康儿童的miRNA表达谱,通过qPCR对微阵列芯片筛选出的差异表达的24个miRNA进行验证。结果与对照组比较,试验组患儿miR-320d、miR-34b、miR-125a和miR-429表达下调,而miR-155、miR-203a和miR-106b表达上调;miR-125a在轻度患儿中的表达水平低于重度患儿(t=6.48,P<0.05)。结论 RSV感染阳性患儿鼻黏膜脱落细胞差异表达miRNA与免疫系统密切相关,而与NF-κB信号通路相关的miR-125a能够解释RSV感染患儿疾病严重程度的差异。

Objective To study the expression of microRNAs in nasal mucosal swab samples of children with respiratory syncytial virus(RSV)infection and its relationship with the severity of symptoms.Methods Twenty-six cases of children with a diagnosis of RSV infection from September 2017 to February 2018 were regarded as experimental group,in which 17 cases were referred as mild subgroup and 9 cases were severe subgroup according their clinical symptoms.Twelve healthy children who came to the hospital for outpatient visits at the same time were selected as the control group.Nasal mucosa specimens were collected,and the microRNAs expression profile for 2 cases of severe subgroup,2 cases of mild subgroup and 2 cases of healthy children were analyzed by microRNAs microarray.24 microRNAs screened out from microRNAs microarray were verified by qPCR.Results Compared with the control group,the expression of miR-320d,miR-34b,miR-125a and miR-429 were down-regulated in the experimental group,while the expression of miR-155,miR-203a and miR-106b were up-regulated.For subgroups analysis,miR-125a expression level in the mild subgroup was significantly lower than that in the severe subgroup(t=6.48,P<0.05).Conclusion The different expression microRNAs in nasal mucosa specimens for RSV positive infants shows a close association with the immune system,especially for miR-125a,which is associated with NF-κB pathway,it might explain the mechanism for difference disease severity in children with RSV infections.