T淋巴细胞亚群在非病毒性肝病中的应用

Application of T-lymphocyte Subsets for Non-viral Liver Disease

目的探讨T淋巴细胞亚群在非病毒性肝病中的应用价值。方法用流式细胞术检测2017年6月1日至2018年5月30日在昆明市第三人民医院收治的180例非病毒性肝病患者外周血T淋巴细胞亚群,与笔者建立的参考区间进行比较分析,有差异的指标进一步用统计学方法分析。结果酒精性肝硬化患者CD3^+、CD3^+CD8^+、CD3^+CD8^+/淋巴细胞低于健康成年人,差异有统计学意义(P<0.05),CD4^+/CD8^+高于健康成年人(P<0.001);原发性胆汁性肝硬化患者CD3^+CD4^+/淋巴细胞、CD4^+/CD8^+高于健康成年人(P<0.001)。对有差异的指标进行ROC曲线分析,在酒精性肝硬化中CD3^+、CD3^+CD8^+、CD3^+CD8^+/淋巴细胞、CD4^+/CD8^+的曲线下面积(AUC)分别为0.80、0.82、0.66、0.74;在原发性胆汁性肝硬化中CD3^+CD4^+/淋巴细胞、CD4^+/CD8+的曲线下面积(AUC)分别为0.80、0.81。结论 T淋巴细胞亚群可以作为酒精性肝硬化和原发性胆汁性肝硬化的辅助诊断指标。

Objective To study the application value of T-lymphocyte subsets for non-viral liver disease Methods180 cases of non-viral liver disease patients in The third people’s Hospital of Kunming from June 1,2017 to May 30,2018 were collected.The peripheral blood of patients were tested and analyzed by BECKMAN Navios Flow Cytometer,and compared with of the reference ranges of peripheral blood T-lymphocyte subsets among healthy adults of Kunming.ROC analysis was used to test each index.Results Compared with the control group,CD3^+,CD3^+CD8^+cells absolute number and the ratio of CD3^+CD8^+/lymphocytes with alcoholic cirrhosis group was significantly lower than the control group(P<0.05),the ratio of CD4^+/CD8^+was significantly higher(P<0.001).The ratio of CD3^+CD4^+/lymphocytes and CD4^+/CD8^+with primary biliary cirr Ilosis group was significantly higher than the control group(P<0.001).The AUC value of CD3^+,CD3^+CD8^+,CD3^+CD8^+/lymphocytes,CD4^+/CD8^+with alcoholic cirrhosis group was 0.80,0.82,0.66,0.74.The AUC value of CD3^+CD4^+/lymphocytes and CD4^+/CD8^+with primary biliary cirr Ilosis group had a greater value,was 0.80,0.81.ConclusionT lymphocyte subset can be the assistant diagnostic index of alcoholic cirrhosis and primary biliary cirrhosis.