结核分枝杆菌利福平耐药突变基因检测与BD960药敏的对比

Comparative Analysis of Rifampicin-resistant Mutant Gene Detection of Mycobacterium Tuberculosis and Drug Sensitivity BD960

目的分析对比结核分枝杆菌利福平PCR-反向点杂交法耐药基因突变位点检测和BACTEC MGIT960 System表型药敏结果的相关性。方法同时对220例住院患者的痰或肺泡灌洗液(BALF)用PCR-反向点杂交法检测利福平耐药基因突变位点与BACTEC MGIT 960 System检测利福平表型药敏结果进行对比分析。结果 2种方法检测利福平药敏结果符合205例,特异度93%(205/220);2种方法检测利福平都敏感的有172例;基因耐药,表型耐药的有33例,其中有3例检出rpo B基因H526位点突变,突变率9%;基因耐药,表型敏感的有15例,其中有11例均检出rpo B基因H526位点突变,突变率73%。结论 PCR-反向点杂交法检测具有快速高效、特异度高等特点,对临床诊断、治疗有指导意义。

Objective To analyze and compare rifampicin-resistant gene mutation detection and BACTEC MGIT 960 system phenotype susceptibility test in Mycobacterium tuberculosis by PCR-reverse dot blot hybridization.Methods At the same time, the phenotypic susceptibility of rifampicin to rifampicin was detected by PCR-reverse dot blot hybridization in sputum or bronchoalveolar lavage fluid(BALF) from 220 inpatients and by BACTEC MGIT 960 System. Results The susceptibility of rifampicin to rifampicin by the two methods accorded with 205 cases and the specificity was 93%(205/220);both methods were sensitive to rifampicin in 172 cases;gene resistance and phenotypic resistance in 33 cases, of which 3 cases detected mutation at the H526 locus of rpo B gene, with a mutation rate of 9%;gene resistance and phenotypic sensitivity in 15 cases,11 of which were all detected. The mutation of the H526 locus of rpo B gene was 73%.Conclusions PCR-reverse dot hybridization has the characteristics of high speed, high efficiency and specificity. It has guiding significance for clinical diagnosis and treatment.