摘要
目的研究云南彝族215个健康无关个体19个常染色体STR基因座的遗传多态性,计算群体遗传学参数,建立云南彝族群体的遗传学基础数据,为法医物证亲权鉴定和个体识别提供科学依据。方法采用Chelex-100法提取样本DNA,Power PlexR21 System试剂盒进行扩增,ABI 3130XL自动遗传分析仪对PCR复合扩增产物进行分析,用ABI的Gene Mapper v3.2软件进行STR基因分型分析,用Modified-Powerstates软件进行法医学遗传学参数统计分析以及Hardy-Weinberg平衡检验。结果共检出203个等位基因和666种基因型。除D1S1656、D5S818、D12S391外,基因型分布均符合Hardy-Weinberg平衡规律(P> 0.05),累积非父排除率(CPE)为0.999 999 907,累积个人识别能力(TDP)为0.999 999 999 999 999 999 999 9。结论 19个常染色体基因座在云南彝族人群中具有较高的多态性和较好的个体识别能力,能够为法医学个体识别和亲权鉴定提供科学的遗传学基础数据。
Objective To study the genetic polymorphisms of 19 autosomal STR loci in 215 healthy unrelated individuals of Yunnan Yi population, calculate population genetic parameters, and establish genetic basis data of Yunnan Yi population, and provide scientific basis for forensic material identification and individual identification.Methods The sample DNA was extracted by Chelex-100 method, amplified by Power Plex 21 System kit, and the PCR composite amplification product was analyzed by ABI 3130 XL automatic genetic analyzer. STR was performed with ABI Gene Mapper v3.2 software. Genotyping analysis, statistical analysis of forensic genetic parameters and Hardy-Weinberg equilibrium test using Modified-Powerstates software. Results A total of 203 alleles and 666 genotypes were detected. Except D1 S1656, D5 S818, D12 S391, the genotype distribution accorded with Hardy-Weinberg equilibrium law(P >0.05), the cumulative non-parent exclusion rate(CPE)was 0.999 999 907, and the cumulative personal recognition ability(TDP) was 0.999 999 999 999 999. 999 9999. Conclusion The 19 autosomal loci have high polymorphism and good individual recognition ability in Yunnan Yi population, and can provide scientific genetic basic data for forensic individual identification and paternity identification.
作者
杨朔
姜焰凌
方宝雄
计艾岑
张寿勋
张秀峰
张柠
赵斐
钟树荣
YANG Shuo;JIANG Yan-ling;FANG Bao-xiong;JI Ai-cen;ZHANG Shou-xun;ZHANGXiu-feng;ZHANG Ning;ZHAO Fei;ZHONG Shu-rong(School of Forensic Medicine,Kunming Medical University,Kunming Yunnan 650500;CriminalInvestigation Brigade,Qinnan Branch of Qinzhou Municipal Public Security Bureau,Guangxi ZhuangAutonomous Region,Qinzhou Guangxi 535037;Judicial Identification Center,Kunming MedicalUniversity,Kunming Yunnan 650500;Judicial Identification Center of Tianjin Dian,Tianjin 300110,China)
出处
《昆明医科大学学报》
CAS
2019年第3期120-125,共6页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81660232
81000577)
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2015FB011)
昆明医科大学百名中青年学术技术骨干项目(60117190413)
昆明医科大学大学生创新性试验计划项目(CX201416
CX201544
CX201654
601162023)
关键词
STR
云南彝族
遗传多态性
STR
Yunnan Yi population
Genetic polymorphism