多孔双相磷酸钙陶瓷修复骨质疏松症大鼠颅骨极量缺损的实验研究

Experimental Study on Repair of Critical-size Calvarial Defects in Osteoporotic Rats by Biphasic Calcium Phosphate Ceramic Scaffold

目的:探讨多孔双相磷酸钙陶瓷支架(BCP)对骨质疏松症SD大鼠颅骨极量缺损的修复效果。方法:建立骨质疏松症SD大鼠颅骨极量缺损模型,运用BCP修复颅骨极量缺损,分为4组,分别是Ctrl组,OP组,Ctrl+BCP组,OP+BCP组,8周后处死大鼠,应用Micro-CT、HE和Masson染色检测骨形成差异。结果:Ctrl组和OP组未见明显新生骨组织;Ctrl+BCP组和OP+BCP组可见新生骨组织,骨质疏松症组(OP+BCP组)新生骨组织明显少于非骨质疏松组(Ctrl+BCP组)。结论:BCP对骨质疏松症SD大鼠颅骨极量缺损具有一定的修复作用,可作为骨质疏松症大鼠颅骨极量缺损修复的支架材料,但修复效果弱于正常SD大鼠。

Objective:To study the effect of the biphasic calcium phosphate ceramic scaffold(BCP)on the repair of critical-size calvarial defects in SD rats with osteoporosis.Methods:SD rats were made into critical-size calvarial defects with osteoporosis.BCP was used to repair the defect of the skull.The rats were divided into four groups,i.e.the Ctrl group,the OP group,the Ctrl+BCP group,and the OP+BCP group.Rats in each group were sacrificed 8 weeks later and the differences in bone formation were detected by imaging findings(Micro-CT)and histological manifestations(Masson staining and hematoxylin-eosin staining).Results:No obvious new bone was found in the Ctrl group and the OP group.New bone can be found in the Ctrl+BCP group and the OP+BCP group,and the new bone in the OP+BCP group was significantly less than that in the non-osteoporosis group(the Ctrl+BCP group).Conclusion:BCP can be used as the scaffold for the repair of critical-size calvarial defects in SD rats with osteoporosis,but the repair effect is weaker than that of normal SD rats.