探究乌司他丁和多西他赛对人乳腺癌细胞MDA-MB-231增殖、侵袭的影响

Effect of ulinastatin and docetaxel on proliferation and invasion of MDA-MB-231 human breast cancer cells

目的探究分析乌司他丁和多西他赛对体外培养的人乳腺癌细胞MDA-MB-231增殖、侵袭和迁移能力的影响。方法将人乳腺癌细胞MDA-MB-231随机分为乌司他丁组、多西他赛组、乌司他丁联合多西他赛组以及对照组四个组,分别予以800 U/ml乌司他丁、3. 7μg/ml多西他赛、800 U/ml乌司他丁+3. 7μg/ml多西他赛以及等量生理盐水干预。分别在干预后0 h、24 h、48 h、72 h时点采用CCK8法检测MDA-MB-231的增殖能力,在干预后48 h采用Transwell法检测MDA-MB-231的侵袭、迁移能力。结果与对照组相比,乌司他丁组、多西他赛组、乌司他丁联合多西他赛组24 h、48 h、72 h等各时点MDA-MB-231的增殖能力均明显降低(P <0. 05),且多西他赛组的增殖能力较同时点乌司他丁组较低(P <0. 05),乌司他丁联合多西他赛组各时点增殖能力则明显降低(P <0. 05),组间比较差异明显,具有统计学意义。与对照组相比,乌司他丁组、多西他赛组、乌司他丁联合多西他赛组各组MDA-MB-231细胞侵袭、迁移能力明显降低(P <0. 05),且乌司他丁联合多西他赛组细胞侵袭、迁移能力明显低于多西他赛组及乌司他丁组水平(P <0. 05),多西他赛组细胞侵袭、迁移能力明显低于乌司他丁组(P <0. 05),组间差异具有显著统计学意义。结论乌司他丁与多西他赛均可在一定程度上抑制人乳腺癌细胞MDA-MB-231的增殖、侵袭和迁移,且乌司他丁和多西他赛联合应用抑制效果优势明显。

Objective To investigate and analyze the effect of ulinastatin and docetaxel on proliferation,invasion and migration of MDA-MB-231 human breast cancer cells.Methods Human breast cancer cells MDA-MB-231 were randomly divided into four groups:ulinastatin group,docetaxel group,ulinastatin combined with docetaxel group and control group,with intervention of 800 U/ml ulinastatin,3.7μg/ml docetaxel,800 U/ml ulinastatin+3.7μg/ml docetaxel and same amount of normal saline,respectively.Proliferation ability of MDA-MB-231 was detected by CCK8 method at 0 h,24 h,48 h and 72 h after intervention.Invasion and migration ability of MDA-MB-231 was detected by Transwell method 48 h after intervention.Results Compared with control group,the other three groups had lower proliferation at each timepoints(P<0.05);proliferation of docetaxel group was lower at same time points compared with ulinastatin group(P<0.05),and combination group had significantly lower proliferation ability(P<0.05).Compared with control group,the other three groups had significantly lower invasion and migration ability(P<0.05).Combination group had even lower invasion and migration ability(P<0.05),and docetaxel group had lower cell invasion and migration ability than ulinastatin group(P<0.05).Conclusion Both ulinastatin and docetaxel can inhibit proliferation,invasion and migration of MDA-MB-231 human breast cancer cells to some extent.