长非编码RNA-UCA1影响红细胞增殖

LncRNA UCA 1 Regulates Erythrocyte Proliferation

造血是一个高度协调、精密调控的过程。在正常造血分化过程中, lncRNA不仅调控造血干/祖细胞自我更新、分化、凋亡等过程,还决定造血谱系分化命运。关于lncRNA在人的不同造血谱系分化中的功能以及作用机制的研究已比较深入,但其在红系分化过程中的功能和机制的研究很少,仍处于建立差异基因表达谱的阶段。现有的研究表明, lncRNA-UCA 1(urothelial cancer associated 1)作为原癌基因与多种癌症的发生、发展、转移、产生化疗耐药性等密切相关。该研究发现,在体外诱导脐带血来源的CD34+干/祖细胞向红细胞分化的过程中,采用慢病毒感染的方法敲降UCA 1的表达抑制了红细胞的增殖及活力,对RNA-seq数据进一步分析发现,降低UCA 1的表达会影响与细胞周期相关基因的表达。

Hematopoietic system is a highly coordinated and precisely regulated process. It has been shown that lncRNAs are highly involved in hematopoietic development, including apoptosis, proliferation,differentiation of hematopoietic stem/progenitor cells and precursors of multi-lineage mature blood cells, which include lymphocytes(B-cell and T-cell), myelocytes(neutrophil, monocyte and macrophage), erythrocytes and megakaryocytes. The function and mechanism of lncRNAs in the different hematopoietic lineages of human were studied deeply. However, few studies have been reported on the function or mechanism of the regulation of lncRNAs in human erythrocyte development. UCA 1 is a long noncoding RNA(lncRNA) aberrantly expressed in a broad range of cancer tissues and cells, involving tumorigenic processes and regulating tumor cell proliferation and metastasis. Here, our results show that UCA 1 depletion inhibits proliferation and cell vitality and further study reveal that cell cycle related genes are affected.