应用模式生物转基因青鳉幼鱼检测奶粉中的雌激素水平

Determination of estrogen in milk powder by using transgenic medaka larval fish model

目的建立模式生物检测奶粉中雌激素水平的检测方法。方法将转基因青鳉鱼交配后收集胚胎并孵化,将乙腈提取的奶粉雌激素样品暴露于幼鱼,24 h后镜下观察荧光表达,根据荧光值推算样品雌激素当量(活性)。结果部分检测样品暴露后,镜下观察到幼鱼发出绿色荧光,经计算,个别样品雌激素当量>60 ng/g。结论应用模式生物转基因青鳉幼鱼可快速、灵敏地筛检具有雌激素活性的样品。

Objective A detective model of estrogen in milk powder was established by using transgenic medaka larval fish model.Methods After mating and hatching,transgenic medaka embryos were collected and treated with estrogen samples which were extracted from milk powder sample by acetonitrile.24 h after treatment,fluorescence expression in medaka larval fish was observed under microscope.Estrogen equivalents(activity)were calculated according to fluorescence intensity.Results After estrogen exposure,fluorescence expression was observed in several sample groups,estrogen equivalents(activity)in several milk powder samples was more than 60 ng/g.Conclusion Estrogen samples can be quickly and sensitively screened by using transgenic medaka larval fish model.