新西兰大白兔佐剂性关节炎模型的评价

Evaluation of adjuvant arthritis model in New Zealand rabbits

目的建立一种简单、可行的兔佐剂性关节炎动物模型,并对其进行鉴定。方法通过注射弗氏完全佐剂建立兔关节炎模型,测量造模前、后24 d致炎侧的足趾周长变化;摄X线片,观察其软组织肿胀及骨性变化;用滑膜组织HE染色方法观察组织病理学变化。结果造模后24 d致炎侧足趾周长较正常组肿胀明显(<0.05);X线片显示,与正常组比较,佐剂性关节炎动物模型组软组织肿胀明显,关节间隙变窄甚至模糊不清,关节面不平整;取材过程中发现模型兔关节腔有明显积液,模型兔足趾部解剖有明显微血管增生和炎症;滑膜组织HE染色后,正常组的滑膜细胞呈现"串珠样"规则排列,模型组的滑膜细胞排列紊乱并出现水肿。结论成功建立了兔佐剂性关节炎动物模型,方法简便,成功率高,为进一步研究提供良好的实验动物模型。

Objective To establish a simple and feasible animal model of rabbit adjuvant arthritis and to identify it. Methods The rabbits were injected complete Freund's adjuvan (CFA) to establish the model of adjuvant arthritis. Adjuvant arthritis (AA) rabbits the perimeters of the hind toe were measured before and after modeling. The changements of soft tissue and bone were assessed by X-rays. Pathological changes were observed by HE staining method in synovial tissue. Results Before modeling, the perimeters of the toe in four groups showed great difference compared with normal group (P<0.05). Through X ray pictures, compared with the normal group, the edema of model group soft tissue was more serious, joint space loss or even obscured, articular surface is not smooth. During the extraction process, it was found that the model rabbit joint cavity had obvious effusion, and the model rabbit toe was dissected with obvious microvascular hyperplasia and inflammation. After the synovial tissue HE staining, the synovial cells in the normal group showed a beaded pattern arrangement, and the synovial cells in the model group were disordered and edema. Conclusion The animal model of rabbit adjuvant arthritis is successfully established, the method is simple and the success rate is high, which provides a good experimental animal model for further research.