青钱柳活性成分对IR-HepG2细胞葡萄糖消耗量及α-葡萄糖苷酶活性的影响

Effects of Cyclocarya paliurus(Batal.) Iljinskaja Extracts on Glucose Consumption of Insulin Resistance HepG2 Cell and Activity of Alpha-Glucosidase

研究青钱柳多糖(CPP)和青钱柳黄酮(CPF)对胰岛素抵抗的人源肝癌细胞(IR-HepG2)葡萄糖消耗量及α-葡萄糖苷酶活性影响。用高浓度胰岛素诱导培养HepG2细胞,建立IRHepG2细胞模型,将IR-HepG2细胞分为模型组、CPP高、中、低剂量组、CPF高、中、低剂量组和二甲双胍组,各组以相应药物孵育24 h,采用葡萄糖试剂盒测定细胞葡萄糖消耗量(△GC),MTT法测定单位细胞葡萄糖消耗量(△GC/OD);以阿卡波糖为阳性对照,比较不同浓度梯度的CPP与CPF对α-葡萄糖苷酶活性影响。与模型组比较,阴性对照组、二甲双胍组、CPP组与CPF组细胞△GC与△GC/OD显著性升高;不同浓度的CPP与CPF对α-葡萄糖苷酶均有抑制作用。提示青钱柳活性提取物降血糖功效与其提高细胞葡萄糖摄取量,抑制α-葡萄糖苷酶活性有关。

The effects of Cyclocarya paliurus(Batal.) Iljinskaja Extracts on glucose consumption of insulin resistance HepG2 cell and activity of alpha-glucosidase is studied. HepG2 cell with high concentration insulin(10 μg/mL) is induced to establish insulin resistance cell model and divided model cell into Cyclocarya paliurus polysaccharide(CPP),Cyclocarya paliurus flavone(CPF) and dimethylbiguanide groups. After 24 h administration,glucose assay is used fit to measure the gross glucose consumption of cells(△GC), glucose consumption of unite cell(△GC/OD) is detected by MTT assay. The inhibitory activity on alpha-glucosidase of CPP and CPF is evaluated,compared with acarbose,in vitro. Compared with model group,△GC and △GC/OD of IR-HepG2 cell is increased by CPF and CPP significantly. CPP and CPF have inhibitory effect on the activity of alpha-glucosidase. Those results indicat that CPF and CPP have effects on increasing the glucose consumption of cell and decreasing the activity of alpha-glucosidase,thus alleviating hyperglycemia.