摘要
目的探讨上调的长链非编码RNA-uc003jsd.1对先天性小耳畸形的表观遗传学影响及其在小耳畸形中的临床意义。方法选取8例中国医学科学院整形外科医院收治的单侧先天性小耳畸形患者,年龄6~14岁,分别提取正常侧和患侧耳廓软骨中的总RNA,反转录获得cDNA后,通过实时荧光定量PCR方法检测uc003jsd.1的表达量,使用SPSS16.0软件分析uc003jsd.1表达的差异性及其表达水平与患者临床特征之间的相关性。结果uc003jsd.1在先天性小耳畸形患者患侧耳软骨中表达水平明显高于同一患者的健侧耳软骨,在患侧耳软骨中的平均值为0.36,健侧为0.21(P=0.015)。结论长链非编码RNA-uc003jsd.1在先天性小耳畸形的残耳软骨中表达明显上调,提示其和先天性小耳畸形的发生发展有一定的关系。
Objective To investigate the expression of long non-coding RNA(lncRNA) uc003jsd.1 in congenital microtia and its clinical significance. Methods Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of lncRNA-NR_028308 in the residual ear cartilage and normal ear cartilage of 8 patients with microtia. Results The expression of uc003jsd.1 was significantly higher in the residual ear cartilage (P=0.015) than paired normal tissues. Conclusions LncRNA-uc003jsd.1 is up-regulated in the cartilage of the congenital microtia, suggesting a certain relationship with the development of congenital microtia.
作者
张玲
林琳
宋宇鹏
潘博
杨庆华
蒋海越
Zhang Ling;Lin Lin;Song Yupeng;Pan Bo;Yang Qingh ua;Jiang Haiyue(Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100144, China)
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2019年第3期285-288,共4页
Chinese Journal of Plastic Surgery
基金
国家自然科学基金(81701926)
中国医学科学院医学与健康科技创新工程(2016-12M-2-001,2016-12M-2-006)
中央高校基本科研业务费专项资助协和青年基金(33320140171,2017320019).
