Ephrin B2上调促进非小细胞肺癌上皮间质转化及转移

Ephrin B2 promotes epithelial mesenchymal transition and metastasis in nonsmall cell lung cancer

目的:探究Ephrin B2上调对非小细胞肺癌(NSCLC)上皮间质转化及转移的影响。方法:选取NSCLC组织和癌旁正常肺组织,免疫组织化学方法检测NSCLC和正常组织中Ephrin B2蛋白阳性表达率。将转染后的细胞分为4组:空白组(Blank组)、阴性对照组(NC组)、Ephrin B2 vector组、sh-Ephrin B2组。qRT-PCR和Western blot检测各组细胞Ephrin B2、Ecadherin、N-cadherin、Vimentin、MMP-2的表达水平。划痕实验检测各组转染后细胞迁移能力。结果:NSCLC组织中Ephrin B2蛋白表达显著高于正常肺组织和癌旁组织(P <0. 05)。与Blank组相比,Ephrin B2 vector组细胞迁移能力上升,Ephrin B2、Ncadherin、Vimentin、MMP-2 mRNA表达上升(P <0. 05),E-cadherin mRNA和蛋白表达水平下降(P <0. 05)。sh-Ephrin B2组细胞迁移能力下降,Ephrin B2、N-cadherin、Vimentin、MMP-2 mRNA和蛋白表达水平下降(P <0. 05),E-cadherin mRNA和蛋白表达水平上升(P <0. 05)。结论:Ephrin B2上调促进非小细胞肺癌上皮间质转化及转移。

Objective: To examine the role of Ephrin B2 in the effect of Transformation and metastasis of epithelial mesenchymal in non-small cell lung cancer(NSCLC).Methods: Lung cancer and paracancerous tissue were selected,expression of Ephrin B2 protein were observed by immunohistochemical technique.The transfected cells were divided into 4 groups: Blank group,negative control(NC) group,Ephrin B2 vector group,sh-Ephrin B2 group.QRT-PCR and Western blot were used to detect the expression levels of Ephrin B2,E-cadherin,N-cadherin,Vimentin and MMP-2 in each group.Scratch test was used to detect cell migration ability after transfection.Results: the expression of Ephrin B2 protein in NSCLC tissue was significantly higher than that in paracancerous tissue(P <0.05).Compared with the Blank group,the cell migration ability of the Ephrin B2 vector group increased and the expression of Ephrin B2,N-cadherin,Vimentin and MMP-2 protein and mRNA increased(P < 0.05).The level of E-cadherin protein and mRNA decreased(P < 0.05).The cell migration ability of sh-Ephrin B2 group decreased,Ephrin B2,N-cadherin,Vimentin,MMP-2 mRNA and protein expression decreased(P < 0.05),E-cadherin mRNA and protein expression increased(P < 0.05).Conclusion: Ephrin B2 can promotes epithelial mesenchymal transition and metastasis in non-small cell lung cancer.