SOCS1/3对TLR3表达和T细胞分化的调控及其在桥本甲状腺炎中的作用被引量：2

Regulation of SOCS1/3 on TLR3 expression and T cell differentiation and its role in Hashimoto's thyroiditis

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摘要目的:探讨细胞信号传导抑制因子1/3(SOCS1、SOCS3,SOCS1/3)对Toll样受体3(TLR3)和T细胞分化的调控及其在桥本甲状腺炎(HT)中的作用。方法:收集已确诊的桥本甲状腺炎患者40例(HT组)及正常体检人群30例(对照组);通过Ficoll密度梯度离心法分离外周血单核细胞(PBMCs),流式细胞术检测调节性T细胞(CD4+CD25+Treg,Treg)、辅助性T细胞17(Th17)及滤泡辅助性T细胞(Tfh),免疫组化法(IHC)检测甲状腺组织中SOCS1/3及TLR3蛋白表达,ELISA检测血浆中SOCS1/3、STAT3蛋白水平,qRT-PCR检测PBMCs中TLR3、SOCS1/3、STAT3、RORγt、Bcl6、Foxp3 mRNA的表达水平。结果:HT组PBMCs中Treg细胞数量降低而Th17细胞、Tfh细胞数量升高(P <0. 05),TLR3、SOCS1/3、Bcl6、RORγt mRNA表达升高,Foxp3、STAT3 mRNA表达降低(P <0. 05); HT组甲状腺组织SOCS1/3蛋白、TLR3蛋白浓度升高(P <0. 05),STAT3蛋白表达降低(P <0. 05)。结论:HT患者SOCS1/3表达升高,可能对HT的TLR3表达及T细胞亚群分化起调控作用。 Objective: To investigate the regulation of SOCS1,SOCS3(SOCS1/3) on Toll like receptors 3(TLR3) and T cell differentiation and its role in Hashimoto’s thyroiditis.Methods: Forty patients with HT(HT group) and thirty healthy controls(Control group) were collected.Peripheral blood mononuclear cells(PBMCs) were isolated by ficoll density gradient centrifugation.The protortions of regulatory T cells(CD4+CD25+Treg,Treg),T helper cell 17(Th17) and Follicular helper T cells(Tfh) were analyzed by flow cytometry.The protein expression of SOCS1/3 and STAT3 in the thyroid tissues were detected by immunohistochemistry(IHC).The protein epxression of SOCS1/3 and STAT3 in plasma were detected by ELISA.qRT-PCR was used to detect the mRNA expression level of TLR3,SOCS1/3,STAT3,RORγt,Bcl6,Foxp3.Results: In patients with HT,the proportion of Treg cells was decreased,while the proportion of Th17 cells and Tfh cells were significantly elevated.The mRNA expressions of TLR3,SOCS1/3,Bcl6 and RORγt in PBMCs were increased,while the mRNA expressions of Foxp3 and STAT3 were decreased(P < 0.05).The expression levels of TLR3 expression and SOCS1/3 protein in thyroid tissue were increased(P < 0.05),STAT3 protein was decreased(P < 0.05).Conclusion:SOCS1/3 in HT patients are significantly elevated and may play a role in regulation of TLR3 expression and T cell differentiation.

作者周希郑应麟俞燕露戴萌袁首杨治芳 ZHOU Xi;ZHENG Ying-Lin;YU Yan-Lu;DAI Meng;YUAN Shou;YANG Zhi-Fang(Department of Endocrinology,Nanchang University First Affiliated Hospital,Nanchang 330006,China)

机构地区南昌大学第一附属医院内分泌科

出处《中国免疫学杂志》 CAS CSCD 北大核心 2019年第7期845-848,共4页 Chinese Journal of Immunology

基金国家自然科学基金(81260125) 江西省教育厅资助项目(GJJ10056) 江西省卫生计生委科技计划(20171024)资助

关键词桥本甲状腺炎 SOCS1/3 TLR3 STAT3 T细胞分化 Hashimoto’s thyroiditis SOCS1/3 TLR3 STAT3 T cell differentiation

分类号 R581.4 [医药卫生—内分泌]

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1孙庆凯,陈占玲,李春华,张秀薇,孙晓玲,陈伟坤,黄秋霞,黄秀娟.白细胞介素6/转化生长因子-β信号异常在桥本甲状腺炎辅助性T细胞17/调节性T细胞失衡中的作用研究[J].中华内分泌代谢杂志,2015,31(4):320-326. 被引量：9

二级参考文献16

1Eschler DC,Hasham A,Tomer Y. Cutting edge: The etiology ofautoimmune thyroid diseases [ J ]. Clin Rev Allergy Immunol, 2011,41(2):190-197.
2Littman DH, Hudensky AY. Thl7 and regulatory T cells in mediatingand restraining inflammation[ J]. Cell, 2010,140(6) :845-858.
3Morris GP, Brown NK, Kong YC. Naturally-existing CD4 ( +) CD25(+ ) Foxp3 (+) regulatory T cells are required for tolerance toexperimental autoimmune thyroiditis induced by either exogenous orendogenous autoantigen[ J]. J Autoimmun, 2009,33(1) :68-76.
4Li D, Cai W, Gu R, et al. Thl7 cell plays a role in the pathogenesis ofHashiraoto;s thyroiditis in patients[ J]. Clin Immunol, 2013,149(3):411420.
5Lee YK,Mukasa R,Hatton RD,et al. Developmental plasticity ofThl7 and Treg cells [ J ] . Curr Opin Immunol, 2009,21 (3): 274-280.
6To KF, Chan MW, Leung WK, et al. Constitutional activation of IL-6-mediated JAK/STAT pathway through hypermethylation of SOCS-1 inhuman gastric cancer cell line[ J]. Br J Cancer, 2004,91 (7) : 1335-1341.
7Niwa Y, Kanda H,Shikauchi Y, et al. Methylation silencing of SOCS-3 promotes cell growth and migration by enhancing JAK/STAT and FAKsignalings in human hepatocellular carcinoma [ J ]. Oncogene,2005,24(42):6406-6417.
8Egwuagu CE. STAT3 in CD4+ T helper cell differentiation andinflammatory diseases[ J]. Cytokine, 2009,47(3) : 149-156.
9Lo PK, Watanabe H, Cheng PC, et al. MethySYBR, a novelquantitative PCR assay for the dual analysis of DNA methylation andCpG methylation density[ J]. J Mol Diagn, 2009,11(5) :400-414.
10Bossowski A,Moniuszko M,Dabrowska M,et al. Lower proportions ofCD4+CD25 ( high) and CD4+ FoxP3, but not CD4+ CD25+ CD127(low) FoxP3+ T cell levels in children with autoimmune thyroid diseases[J]. Autoimmunity, 2013,46(3) :222-230.