采样参数设置对小鼠心电图波形的影响

Effects of sampling parameters on electrocardiogram waveforms in mice

目的探讨采样参数设置对小鼠心电图波形的影响。方法选用C57小鼠,戊巴比妥钠麻醉,四肢皮下插针式电极,连接RM6240BD型多道生理信号采集处理系统,选择不同的采样参数设置,对心电图进行采样、记录和分析。结果采样速率、低通滤波、时间常数和陷波滤波等采样参数设置均对心电图波形有影响,部分参数会导致心电图不同形式的变形和失真。结论小鼠的心电图实验中应选择合适的采样参数设置,通过操作技术的改进来提高心电图的稳定性,尽量避免或减少滤波的使用,以获得准确的心电图实验结果。

Objective To evaluate the effects of sampling parameters on electrocardiogram (ECG) waveforms in mice. Methods C57 mice were anesthetized with sodium pentobarbital and needle electrodes were placed subcutaneously in their limbs. ECG signals were sampled, recorded and analyzed with different sampling parameters using a RM6240BD multi-channel physiological recording system. Results The sampling rate, low-pass filter, time constant, and notch filter had different effects on ECG waveforms. Some parameters led to various deformation and distortion of the ECG signals. Conclusions Appropriate sampling parameters should be applied in ECG experiments in mice to obtain accurate results. An improved surgical technique is preferred to acquire stable ECG signals instead of filtering or digital smoothing.