鸡源H9N2亚型禽流感病毒感染鸡鸭和小鼠后对血液指标的影响

Effects of Chicken Source H9N2 Subtype Avian Influenza Virus on Blood Indexes of Chickens,Ducks and Mice After Infection

流感可以跨种属传播,通过了解地方性低致病禽流感H9N2亚型病毒感染不同种属动物后的血液变化情况,可为研究禽流感病毒的致病机制提供参考依据。选用52d黑羽乌骨鸡24只,52d樱桃谷鸭24只,6周龄～8周龄昆明系小鼠24只分成3组,攻毒前作为0d空白对照,鸡、鸭和小鼠采用静脉注射感染H9N2亚型流感病毒,分别在感染前(0d)、感染后3d、6d、9d采样,用PE-6800VET三分类动物血细胞分析仪测定血液常规指标。通过对不同种类白细胞和血小板数量变化的分析,结果表明,低致病性禽流感病毒H9N2可以跨种属感染,鸭炎性反应最小,小鼠炎性反应最严重。

Avian influenza virus can spread across species.After the local low pathogenic avian influenza subtype H9N2 virus infecting different species of animals,the changes of their blood can provide a reference for the pathogenic mechanism of avian influenza virus.Twenty four fifty-two days old dark feather silky chickens,twenty four fifty-two days old cherry valley ducks and twenty four six to eight weeks old Kunming mice were divided into three groups.Before injecting the virus as 0 day blank control,chickens,ducks and mice were infected with the H9N2 subtype influenza virus by intravenous injection.The blood was collected and hematologic indexes were measured by the PE-6800 VET animal blood cell analyzer 0 day before infection,3 days,6 days and 9 days after infection.The results showed that low pathogenic avian influenza virus H9N2 can spread across the species.Ducks inflammatory response was minimal and the inflammatory response of mice was most serious in the animals.