结核分枝杆菌感染RAW264.7巨噬细胞MALAT1的表达及其作用研究

Expression and role of MALAT1 in RAW264.7 macrophages infected by Mycobacterium tuberculosis

目的探讨结核分枝杆菌感染RAW264.7巨噬细胞肺腺癌转移相关转录本1(MALAT1)的表达及其作用。方法 H37Rv感染RAW264.7巨噬细胞系,分别在感染后的0、48h后收集细胞,RT-qPCR检测细胞内MALAT1和肿瘤坏死因子-α(TNF-α)mRNA表达。通过siRNA干扰技术沉默巨噬细胞MALAT1表达,检测H37Rv感染细胞后TNF-αmRNA表达及MALAT1表达对H37Rv杀菌功能影响。结果 H37Rv感染巨噬细胞后MALAT1和TNF-αmRNA水平明显上升(P<0.05)。沉默MALAT1的巨噬细胞感染H37Rv后TNF-αmRNA表达明显上调(P<0.01),结核菌清除能力明显增强(P<0.05)。结论 H37Rv感染RAW264.7巨噬细胞可促进MALAT1表达上调,沉默MALAT1可增强巨噬细胞对H37Rv清除能力。

Objective To investigate the expression and role of MALAT1 in RAW264.7 macrophages after H37 Rv Mycobacterium tuberculosis(MTB)infection.Methods H37 Rv was infected to RAW264.7 macrophage cell line,and the cells were collected at 0,48 hafter infection.Intracellular MALAT1 and TNF-αmRNA expressions were detected by RT-qPCR.By using siRNA interference technique,MALAT1 was silenced in macrophages,and after H37 Rv infection to the cells,the expression of TNF-αmRNA in macrophages and the influence of MATAT1 expression on H37 Rv bactericidal function were detected.Results The levels of MALAT1 and TNF-αmRNA after macrophage infection by H37 Rv were significantly increased(P<0.05).By silencing MALAT1 in macrophage infection by H37 Rv,the TNF-αmRNA expression was significantly up-regulated(P<0.01),and the ability of MTB scavenging capacity was significantly increased(P<0.05).Conclusion H37 Rv infecting RAW264.7 macrophages can promote MALAT1 expression up-regulation,and silencing MALAT1 can enhance the scavenging ability of macrophages to intracellular H37 Rv.