摘要
本研究以从竹荪中分离纯化并经不同截留分子量的透析袋透析所得到的DI-1和DI-2为研究对象,借助傅里叶变换红外光谱技术(FTIR)和核磁共振波谱法(NMR)对其结构进行研究。结果表明,FIIR光谱显示,不同截留分子量的透析袋所得DI-1和DI-2的多糖结构明显,无其他结构,为多糖纯品,其单糖是以吡喃糖苷键的形式存在,但DI-1和DI-2具有不同的空间结构;1HNMR谱结果显示,DI-1由4种单糖构成,DI-2由3种单糖组成。上述结果表明,不同截留分子量的透析袋会得到结构不同的多糖。
In this study,DI-1 and DI-2 were isolated and purified from Dictyophora indusiata,and they were dialyzed in dialysis bags with different molecular weight interception. Fourier transform infrared spectroscopy(FTIR) and nuclear magnetic resonance spectroscopy(NMR) were used to analyze its structure. The DI-1 and DI-2 obtained from dialysis bags with different molecular weight interception had obvious polysaccharides structure,and no other structure. They were pure polysaccharides,and the monosaccharides existed in the form of pyranoside,but they had different spatial structures. The1 HNMR spectra showed that DI-1 was composed of four kinds of monosaccharides and DI-2 was composed of three kinds of monosaccharides. The results indicated that polysaccharides with different structures could be obtained from dialysis bags with the different molecular weight interception.
作者
朱淼
侯怡铃
唐贤
宋志强
丁祥
ZHU Miao;HOU Yi-ling;TANG Xian;SONG Zhi-qiang;DING Xiang(College of Life Sciences,China West Normal University/Key Laboratory of Southwest China Wildlife Resources Conservation,Nanchong Sichuan 637009;College of Environmental Science and Engineering,China West Normal University)
出处
《现代农业科技》
2019年第7期206-207,220,共3页
Modern Agricultural Science and Technology
基金
四川省科技厅应用基础重点项目(2018JY0087)
四川省科技厅重点研发项目(2018NZ0055)
四川省教育厅重大培育项目(16CZ0018)
南充市科技局项目(16YFZJ0043)
关键词
竹荪多糖
透析袋
傅里叶变换红外光谱技术(FTIR)
核磁共振波谱法(NMR)
Dictyophora indusiata polysaccharide
dialysis bag
fourier transform infrared spectroscopy(FTIR)
nuclear magnetic resonance spectroscopy(NMR)
