SlSP5G3基因对‘Micro-Tom’番茄开花和产量的影响

Effects of SlSP5G3 Gene on Flowering and Yield of ‘Micro-Tom’ Plants

番茄(Solanum lycopersicum L.)开花时间直接决定后期的产量和经济效益。为了探讨番茄 SlSP5G3基因功能,以‘Micro-Tom’番茄为试验材料,观察 SlSP5G3基因时间和空间的变化规律及对番茄开花和产量的影响。结果发现 SlSP5G3的表达响应光周期变化,主要在叶片中表达,且表达量不受生长发育时期的影响。在‘Micro-Tom’番茄中过表达 SlSP5G3,获得过表达植株L8和L12。与野生型植株相比,L8和L12植株开花所需叶片数分别增加2.34和2.67片;叶片鲜质量分别增加98.43%和76.89%;叶片干质量分别增加62.79%和41.86%;果实单果质量分别增加49.65%和36.13%;单株产量分别增加34.50%和26.74%,并且 SlSP5G3过表达植株还显著提升了上游开花转录因子 SlCDF3的表达量。 SlSP5G3通过调控开花时间,改变植株生长形态,对提升植株产量产生重要影响。

The flowering time of tomato(Solanum lycopersicum L.) directly determines the yield and economic benefits. In order to investigate the effect of tomato SlSP5 G3 gene on flowering,the expression of SlSP5 G3 was observed by using ‘Micro-Tom’ tomato as experimental material. The results showed that SlSP5 G3 mainly expressed in leaves,which responded to photoperiod changes but the expression level was not affected by the growth and development period. Compared with the wild-type plants,the number of leaves required for flowering increased by 2.34 and 2.67 in overexpressing plants L8 and L12,respectively;the fresh mass of leaves increased by 98.43% and 76.89%,respectively;the dry mass of leaves increased by 62.79% and 41.86% respectively. The per fruit mass increased by 49.65% and 36.13%,respectively;the yield per plant increased by 34.50% and 26.74%,respectively. In attention,the over-expressing plants of SlSP5 G3 also significantly increased the expression of the upstream flowering transcription factor SlCDF3. Thus, SlSP5 G3 has an important influence on plant yield by regulating flowering time and changing plant growth morphology.