微波提取白茯苓美白成分的工艺研究

Microwave-assisted extraction technology of skin-whitening agent in white Poria cocos

以中药白茯苓为研究对象,微波辅助提取其美白成分。在提取溶剂、液料比、提取时间、微波功率单因素试验基础上,利用Box-Behnken响应面设计模型,以提取物对酪氨酸酶的相对抑制率为评价标准,应用响应面分析优化,找出了各个因素的最优组合及响应值的最佳值。确定最佳的提取工艺条件为:以水为提取溶剂,液料比为10∶1(mL:g),微波功率520W,微波时间7min,提取2次。在该条件下提取物得率为12.70%,对酪氨酸酶的相对抑制率为31.10%,与模型理论值31.33%很接近。

The microwave-assisted extraction of skin-whitening agent from white Poria cocos was optimized by response surface methodology.Based on single factor experiment,the extraction solvent,liquid to solid ratio,extraction time,and microwave power were selected as independent variables and the relative inhibition rate of the extractive against tyrosinase was used as the response value.The Box-Behnken design and response surface method were used to optimize the extraction technology.The optimized conditions of extraction are as follows:distilled water is used as extraction solvent,with liquid to solid ratio of 10∶1(mL∶g),extraction time of 7 min,microwave power of 520 W,and being extracted twice in one process.Under the optimized conditions,the yield of the extractive is 12.70%,and the relative inhibition rate is 31.10%,which is close to the theoretically predicted value(31.33%).